Which of the following stain is not used for staining chromosomes?
1. Basic fuchsin
2. Safranin
3. Methylene blue
4. Carmine
(2) Safranin It is used as a counterstain in gram staining and endospore staining. It can also be used for detection of cartilage, mucin and mast cell granule. Carmine The basic dye, is used to stain nucleic acid and chromosomes, which possess negative charge on them. It gives chromosomesa pink colour thus,
differentiating from other cellular organelles Basic Fuchsin It involves in staining of human chromosomes elastic fibres, cardiac or skeletal muscle tissue.
Methylene blue It used to stain nuclei, Golgi bodies and pectic substances.
