Q. 34 A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA b and, while linear DNA shows
two fragments. Explain.
Plasmid is a circular DNA molecule and when cut with these enzyme, it becomes linear but does not gel faymeniled due to presence of only one restriction site, whereas a linear DNA molecule gets cut into two fragment.
© 2024 GoodEd Technologies Pvt. Ltd.