Q. 34 A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA b and, while linear DNA shows
two fragments. Explain.


When a plasmid DNA and a linear DNA having one site for a restriction endonuclease are cut and separated, plasmid shows one DNA band, while linear DNA shows two band because of difference in their basic structure.

Plasmid is a circular DNA molecule and when cut with these enzyme, it becomes linear but does not gel faymeniled due to presence of only one restriction site, whereas a linear DNA molecule gets cut into two fragment.