Q. 40 Identify and explain A, B and C in the PCR diagram given below.
In PCR, each cycle has three steps
(i) Denaturation of DNA Sample Unwinding of two strand of DNA by heating the sample at 92-94°C.
(ii) Primer Annealing Primers get positioned on the exposed nucleotides as per base Pairing rules.
(iii) Extension of Pruners DNA polymerase recognises primers as ‘start’ tags and begins to extend the primers using the free nucleotides provided in the reaction and the genomic DNA as template. With each round of reactions, the DNA doubles.
© 2024 GoodEd Technologies Pvt. Ltd.
