During skeletal muscle contraction, the thick filament slides over the thin filament by a repeated binding and releases myosin along the filament. This whole process occurs in a sequential manner.
Step 1: Muscle contraction is initiated by signals that travel along the axon and reachthe neuromuscular junction or motor end plate. Neuromuscular junction is a junctionbetween a neuron and the sarcolemma of the muscle fibre. As a result, Acetylcholine (a neurotransmitter) is released into the synaptic cleft by generating an action potential in sarcolemma.
Step 2: The generation of this action potential releases calcium ions from the sarcoplasmic reticulum in the sarcoplasm.
Step 3: The increased calcium ions in the sarcoplasm leads to the activation of actin sites. Calcium ions bind to the troponin on actin filaments and remove the tropomyosin, wrapped around actin filaments. Hence, active actin sites are exposed and this allows myosin heads to attach to this site.
Step 4: In this stage, the myosin head attaches to the exposed site of actin and forms cross bridges by utilizing energy from ATP hydrolysis. The actin filaments are pulled. As a result, the H-zone reduces. It is at this stage that the contraction of the muscle occurs.
Step 5: After muscle contraction, the myosin head pulls the actin filament and releases ADP along with inorganic phosphate. ATP molecules bind and detach myosin and the cross-bridges are broken.
Stage 6: This process of formation and breaking down of cross bridges continues until there is a drop in the stimulus, which causes an increase in calcium. As a result, the concentration of calcium ions decreases, thereby masking the actin filaments and leading to muscle relaxation.
