During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
1.
Histones
2.
Polysaccharides
3.
RNA
4.
DNA
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain,
| 1. | it will lead to the lysis of host cells. |
| 2. | it will be able to produce a novel protein with dual abilities. |
| 3. | it will not be able to confer ampicillin resistance to the host cell. |
| 4. | the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside. |
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1. Palindromic Nucleotide sequences
2. Poly(A) tail sequences
3. Degenerate primer sequence
4. Okazaki sequences
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
| 1. | Denaturation | 2. | Ligation |
| 3. | Annealing | 4. | Extension |
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
1. Extension, Denaturation, Annealing
2. Annealing, Denaturation, Extension
3. Denaturation, Annealing, Extension
4. Denaturation, Extension, Annealing
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
| 1. | Dark red bands | 2. | Bright blue bands |
| 3. | Yellow bands | 4. | Bright orange bands |
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents.
| 1. | A - Denaturation at a temperature of about 50ºC |
| 2. | C - Extension in the presence of heat-stable DNA polymerase |
| 3. | A - Annealing with two sets of primers |
| 4. | B - Denaturation at a temperature of about 98ºC separating the two DNA strands |
Select the correct statement from the following:
| 1. | Gel electrophoresis is used for the amplification of a DNA segment. |
| 2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
| 3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
| 4. | PCR is used for the isolation and separation of genes of interest. |
Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
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