Normally, microinjection and biolistics [gene gun] are used, respectively, to transform:
1. plant cells and animal cells
2. animal cells and plant cells
3. animal cells and bacterial cells
4. plant cells and bacterial cells
Subtopic: Transforming Plant & Animal Cell |
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It is usually necessary to induce ‘competence’ in bacterial cells to transform them in recombinant DNA procedures mainly because:
| 1. | bacterial cell has a cell wall |
| 2. | bacteria do not have a well defined nucleus and membrane bound organelles |
| 3. | DNA is a hydrophilic molecule |
| 4. | DNA is associated with positively charged histone proteins |
Subtopic: Tools |
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It is possible to make human proteins in bacterial cells with rDNA experiments because:
| 1. | bacterial cells replicate faster than eukaryotic cells |
| 2. | genetic code is almost universal |
| 3. | bacterial cells have plasmids that can be used as a vector |
| 4. | bacterial cells produce restriction enzymes |
Subtopic: General Design of an rDNA experiment |
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Polymerase chain reaction can amplify DNA, producing about 1 billion copies in 30 cycles. The correct sequence of the steps of this reaction is:
| 1. | Denaturation → Annealing of primers → Extension of primers |
| 2. | Denaturation → Extension of primers → Annealing of primers |
| 3. | Extension of primers → Annealing of primers → Denaturation |
| 4. | Annealing of primers → Extension of primers → Denaturation |
Subtopic: Polymerase Chain Reaction: PCR |
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Taq polymerase is:
| 1. | is a ribozyme involved in RNA splicing in eukaryotes |
| 2. | is a thermostable DNA polymerase used in PCR |
| 3. | is a key reagent in ELISA |
| 4. | an enzyme isolated from Thermus aquaticus, a fungus found in damp soils |
Subtopic: Polymerase Chain Reaction: PCR |
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Identify the incorrect statement:
| 1. | The process of separation and purification of expressed protein before marketing is called as downstream processing |
| 2. | DNA precipitation out of a mixture of biomolecules can be achieved by treatment with a divalent cation such as calcium ion |
| 3. | Stirred-tank bioreactors have been designed for availability of oxygen throughout the process |
| 4. | Reporter genes can be used as markers for selecting successfully transformed cells |
Subtopic: Process of Biotech |
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The first restriction endonuclease, whose functioning depended on a specific DNA nucleotide sequence was:
| 1. | EcoR I | 2. | BamH I |
| 3. | Hind II | 4. | Sma I |
Subtopic: Restriction Enzymes - Main Enzymes |
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R)
In the light of the above statements, choose the correct answer from the options given below:
| Assertion (A): | Polymerase chain reaction is used in DNA amplification. |
| Reason (R): | The ampicillin resistant gene is used as a selectable marker to check transformation. |
| 1. | (A) is not correct but (R) is correct. |
| 2. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 3. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
| 4. | (A) is correct but (R) is not correct. |
Subtopic: Selectable Markers: I | Polymerase Chain Reaction: PCR |
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Which one of the following statement is not true regarding gel electrophoresis technique?
| 1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
| 2. | The process of extraction of separated DNA strands from gel is called elution. |
| 3. | The separated DNA fragments are stained by using ethidium bromide. |
| 4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
Subtopic: Separation and Isolation of DNA fragments |
85%
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NEET - 2022
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In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?
1. 5'GTATTC3' ; 3'CATAAG5'
2. 5'GATACT3' ; 3'CTATGA5'
3. 5'GAATTC3' ; 3'CTTAAG5'
4. 5'CTCAGT3' ; 3'GAGTCA5'
Subtopic: Restriction Enzymes - Main Enzymes |
93%
From NCERT
NEET - 2022
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