Select Question Set:
Q. No.Rearrange the following in correct sequence to accomplish a successful polymerase chain reaction
A.
Denaturation of ds‐DNA to genomic DNA tempelate
B.
Thermostable DNA‐polymerase to enzyme DNA‐polymerase
C.
In vitro synthesis of region of DNA of interest
D.
Synthesis of oligonucleotides by primers
E.
Nucleotides complementary region of DNA
Choose the correct answer from the options given below
1. A, B, C, D, E
2. A, D, B, E, C
3. A, C, D, B, E
4. A, D, C, B, E
Subtopic: Polymerase Chain Reaction: PCR |
Please attempt this question first.
Hints
Please attempt this question first.
Melting of DNA at 94°C is due to breakdown of
1. Glycosidic bonds
2. Phosphodiester bonds
3. Disulphide bonds
4. Hydrogen bonds
1. Glycosidic bonds
2. Phosphodiester bonds
3. Disulphide bonds
4. Hydrogen bonds
Subtopic: Polymerase Chain Reaction: PCR |
58%
From NCERT
Please attempt this question first.
Hints
Please attempt this question first.
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule
\(5'C-T-G-C-A \downarrow G3\\ 3'G-A-C-G-T \downarrow C5'\\ ~~~~~~\uparrow\)
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I
\(5'C-T-G-C-A \downarrow G3\\ 3'G-A-C-G-T \downarrow C5'\\ ~~~~~~\uparrow\)
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I
| 1. | \(5'C-T-G~\\ 3'-G-A-C-G-T'\) | \(C-A-G3'\\ C'5\) |
| 2. | \(5'C-T\\ 3'G-A-G-C \) | \(G-C-A-G3'\\ T-C'5\) |
| 3. | \(5'C-T-G-C \\ 3'G-A-C-G \) | \(A-G3'\\ T'-C5'\) |
| 4. | \(5'C-T-G-C-A\\ 3'G\) | \(3'G\\ A-C-G-T-C'5\) |
Subtopic: Restriction Enzymes - Main Enzymes |
55%
Please attempt this question first.
Hints
Assertion(A): Synthetic oligonucleotide polymers are used during Annealing in a PCR
Reason(R): The primers bind to the double stranded DNA at their complementary regions
Reason(R): The primers bind to the double stranded DNA at their complementary regions
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are true, but (R) is not the correct explanation of (A) |
| 3. | (A) is true, but (R) is false |
| 4. | (A) is false, but (R) is true |
Subtopic: Polymerase Chain Reaction: PCR |
From NCERT
Please attempt this question first.
Hints
Please attempt this question first.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
1. i-S, ii-R, iii-Q
2. i-R, ii-S, iii-P
3. i-Q, ii-R, iii-P
4. i-P, ii-R, iii-S
| Column I (PCR steps) |
Column II (Temperature) |
||
| i. | Polymerization | P. | \(40-60 ^\circ C\) |
| ii. | Denaturation | Q. | \(70-75^ \circ C\) |
| iii. | Primer annealing | R. | \(90-98 ^ \circ C \) |
| S. | \(28-37^ \circ C\) |
2. i-R, ii-S, iii-P
3. i-Q, ii-R, iii-P
4. i-P, ii-R, iii-S
Subtopic: Polymerase Chain Reaction: PCR |
64%
Please attempt this question first.
Hints
Please attempt this question first.
From the following identify the INCORRECT pair.
| Restriction enzyme | Source | |
| 1. | AluI | Arthrobacter luteus |
| 2. | EcoRI | Esherichia coli Ry13 |
| 3. | BamHI | Bacillus thuringiensis H |
| 4. | HindII | H. influenza Rd |
Subtopic: Tools: Vectors: I |
59%
Please attempt this question first.
Hints
Please attempt this question first.
From the following identify the INCORRECT pair.
| Restriction enzyme | Type of ends produced | |
| 1. | AluI | Blunt ends |
| 2. | EcoRI | Sticky ends |
| 3. | BamHI | Sticky ends |
| 4. | HindII | Sticky ends |
Subtopic: Restriction Enzymes - Main Enzymes |
Please attempt this question first.
Hints
Please attempt this question first.
Match the following enzymes with their functions:
Select the correct option from the following:
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
| Column I | Column II | ||
| i. | Restriction endonuclease | a. | Joins the DNA fragments |
| ii. | Restriction exonucleases | b. | Extends primers on genomic DNA template |
| iii. | DNA ligase | c. | Cuts DNA at Specific position |
| iv. | Taq polymerase | d. | Removes nucleotides from the ends of DNA |
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
82%
From NCERT
Please attempt this question first.
Hints
Please attempt this question first.
Study the following lists:
1. (i-c); (ii-e); (iii-d); (iv-b)
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
| List I | List II | ||
| i. | Vector | a. | Resistant to cotton bollworms. |
| ii. | Downstream processing | b. | Mobile genetic elements |
| iii. | Cry II AB | c. | Controls corn borer |
| iv. | Transposons | d. | Ti plasmid |
| e. | Purifying protein in biopharmaceuticals. |
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
Subtopic: Bt Crops |
82%
From NCERT
Please attempt this question first.
Hints
Please attempt this question first.
Match the following.
The CORRECT answer is
| List I | List II | List II | |||
| i. | Hind III | a. | Agarose gel | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marker |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequences | 4. | Transgenic plant |
| i | ii | iii | iv | |
| 1. | d,1 | a,3 | c,3 | b,4 |
| 2. | d,1 | c,2 | a,3 | b,4 |
| 3. | d,1 | c,2 | b,4 | a,3 |
| 4. | a,3 | b,4 | c,2 | d,1 |
Subtopic: Tools: Vectors: I |
74%
Please attempt this question first.
Hints
Please attempt this question first.
Select Question Set:
© 2024 GoodEd Technologies Pvt. Ltd.