Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be:

A. Denatured double stranded DNA probes

B. doubles stranded RNA probes

C. Protein probes

D. Single stranded DNA probes.

(1) A, B

(2) B, C

(3) A, D

(4) A, B, C, D

Which is a natural genetic engineer of the plants?

(1)E.coli

(2)A. tumefaciens

(3)Rhizobium

(4)Pseudomonas

The thermostable enzyme Taq polymerase used in PCR technique was discovered by

(1)Alec Jeffrey

(2)Karry Mullis

(3)Temin

(4)Baltimore

To be useful in genetic engineering a vector must have

1. An origin for replication
2. Low copy number
3. Low rates of multiplication
4. Many cloning sites for the same enzyme

In 1960’s two enzymes were discovered in bacteria that were responsible for providing immunity against bacteriophages. One was Restriction Endonuclease and the other was

1. Methylase

2. Exonuclease

3. Aminotransferase

4. Terminal Transferase

 E.coli is  a commonly used host for gene cloning because

1. It is free from elements that interfere with replication and recombination of DNA

2. It is easy to transform

3. It supports replication of inserted DNA.

4. All of these

A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called

a.  Suppression vector

b. Expression vector

c. Cloning vector

d. None of these

Electroporation facilitates insertion of foreign DNA into the cell by

1. Changing the porocity of the membrane

2. Changing the electrical potential of the membrane

3. Lysing the cell wall

4. Active transport

The names associated with construction of first Recombinant DNA are

a. Arber, Nathans, Smith

b. Annie Chang, Boyer, Berg, Cohen

c. Howard Temin, Brenner, Sharp

d. Tim Hunt, Hartwell, Nurse

The restriction enzymes most commonly used in rDNA technology are

1. Type 1 Restriction enzymes

2. Type II Restriction enzymes

3. Type III Restriction enzymes

4. Type IV Restriction enzymes