List I | List II | ||
i. | Vector | a. | Resistant to cotton bollworms. |
ii. | Downstream processing | b. | Mobile genetic elements |
iii. | Cry II AB | c. | Controls corn borer |
iv. | Transposons | d. | Ti plasmid |
e. | Purifying protein in biopharmaceuticals. |
List I | List II | List II | |||
i. | Hind III | a. | Agarose gel | 1. | Six base pairs |
ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marker |
iii. | T-DNA | c. | Ampicillin | 3. | Elution |
iv. | DNA | d. | Recognition sequences | 4. | Transgenic plant |
i | ii | iii | iv | |
1. | d,1 | a,3 | c,3 | b,4 |
2. | d,1 | c,2 | a,3 | b,4 |
3. | d,1 | c,2 | b,4 | a,3 |
4. | a,3 | b,4 | c,2 | d,1 |
List I | List II | ||
1 | Eco RI | I | Vector for transgenic plants |
2 | Ti plasmid | II | Restriction enzyme |
3 | pBR 322 | III | Probe |
4 | Reverse transcriptase | IV | RNA dependent DNA synthesis |
V | Artificial plasmid |
1 | 2 | 3 | 4 | |
1. | II | I | V | IV |
2. | I | II | III | IV |
3. | II | I | IV | V |
4. | II | I | IV | III |
Column I | Column II | ||
(a) | Gel electrophoresis | (i) | Chimeric DNA |
(b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
(c) | rDNA technology | (iii) | Palindrome |
(d) | PCR | (iv) | Separation of DNA fragments. |
Column-I | Column-II | ||
i. | Alu I | a. | Bacillus amyloliquefaciens H |
ii. | Bam HI | b. | H. influenza RD |
iii. | Eco RI | c. | Arthobacter luteus |
iv. | Hind II | d. | Escherichia coli Ry 13 |
Column I (Restriction Enzyme) |
Column II (Recognition sequence) |
||
i. | AluI | P. | \(5'---G- \downarrow-A-T-C-C---3'\\3'---C-C-T-A-G-\uparrow-G---5'\) |
ii, | BamHI | Q. | \(5'---A-G-\downarrow-C-T---3'\\ 3' ---T-C- \uparrow -G-A---5' \) |
iii. | EcoRI | R. | \(5'--G-T-C- \downarrow - G-A-C--3' \\ 3'--C-A-G-\uparrow-C-T-G--5'\) |
iv. | HindII | S. | \(5'---G- \downarrow-A-A-T-T-C---3'\\ 3'--- C-T-T-A-A-\uparrow-G---5'\) |
Column-I | Column-II | ||
(a) | DNA fragmentation | (i) | ssDNA gets embedded in nylon membrane |
(b) | Electrophoresis | (ii) | Sample DNA and probe DNA from double stranded DNA |
(c) | Southern blotting | (iii) | DNA fragmentation move to positive pole |
(d) | Hybridization | (iv) | Fragments of DNA with variable length are formed |
Column-I | Column-II | ||
(a) | Gel electrophosis | (i) | To combine genes from two organisms |
(b) | cDNA | (ii) | To make a copy of DNA from mRNA |
(c) | Genetic Engineering | (iii) | Separate the DNA fragments |
(d) | PCR | (iv) | Gene multiplication in vitro |
Column-I | Column-II | ||
(a) | Heat denaturation | (i) | 70-75°C |
(b) | Annealing | (ii) | 90-98°C |
(c) | Primer extension | (iii) | 40-60°C |
Column-I | Column-II | ||
(a) | Amplifications of gene of interest | (i) | Downstream processing |
(b) | Large scale production of plants in short duration | (ii) | Polymerase chain reaction |
(c) | Separation and purification of desired product | (iii) | Transformation |
(d) | Insertion of rDNA into host | (iv) | Micro propagation |