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Q. No.Match the following.
List I
List II
List II
i.
Hind III
a.
Agarose gel
1.
Six base pairs
ii.
pBR322
b.
Agrobacterium
2.
Selectable marker
iii.
T-DNA
c.
Ampicillin
3.
Elution
iv.
DNA
d.
Recognition sequences
4.
Transgenic plant
The CORRECT answer is
i
ii
iii
iv
1.
d,1
a,3
c,3
b,4
2.
d,1
c,2
a,3
b,4
3.
d,1
c,2
b,4
a,3
4.
a,3
b,4
c,2
d,1
Subtopic: Tools: Vectors: I |
74%
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Study the following lists
| List I | List II | ||
| 1 | Eco RI | I | Vector for transgenic plants |
| 2 | Ti plasmid | II | Restriction enzyme |
| 3 | pBR 322 | III | Probe |
| 4 | Reverse transcriptase | IV | RNA dependent DNA synthesis |
| V | Artificial plasmid |
| 1 | 2 | 3 | 4 | |
| 1. | II | I | V | IV |
| 2. | I | II | III | IV |
| 3. | II | I | IV | V |
| 4. | II | I | IV | III |
Subtopic: Tools: Vectors: I |
71%
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Match the technique in columns-I with the terms given in column-II.
1. (a)-(iv), (b)-(ii), (c)-(iii), (d)-(i)
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
| Column I | Column II | ||
| (a) | Gel electrophoresis | (i) | Chimeric DNA |
| (b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
| (c) | rDNA technology | (iii) | Palindrome |
| (d) | PCR | (iv) | Separation of DNA fragments. |
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
Subtopic: Tools |
75%
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Match the restriction enzyme given in Column-I with their source given in Column-II and choose the correct option.
1. i-a, ii-c, iii-d, iv-b
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
| Column-I | Column-II | ||
| i. | Alu I | a. | Bacillus amyloliquefaciens H |
| ii. | Bam HI | b. | H. influenza RD |
| iii. | Eco RI | c. | Arthobacter luteus |
| iv. | Hind II | d. | Escherichia coli Ry 13 |
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
77%
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Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and Select the correct option.
1. (i)-(R),(ii)-(P), (iii)-(S), (iv)-(Q)
2. (i)-(S),(ii)-(P), (iii)-(Q), (iv)-(R)
3. (i)-(P),(ii)-(Q), (iii)-(S), (iv)-(R)
4. (i)-(Q),(ii)-(P), (iii)-(S), (iv)-(R)
| Column I (Restriction Enzyme) |
Column II (Recognition sequence) |
||
| i. | AluI | P. | \(5'---G- \downarrow-A-T-C-C---3'\\3'---C-C-T-A-G-\uparrow-G---5'\) |
| ii, | BamHI | Q. | \(5'---A-G-\downarrow-C-T---3'\\ 3' ---T-C- \uparrow -G-A---5' \) |
| iii. | EcoRI | R. | \(5'--G-T-C- \downarrow - G-A-C--3' \\ 3'--C-A-G-\uparrow-C-T-G--5'\) |
| iv. | HindII | S. | \(5'---G- \downarrow-A-A-T-T-C---3'\\ 3'--- C-T-T-A-A-\uparrow-G---5'\) |
1. (i)-(R),(ii)-(P), (iii)-(S), (iv)-(Q)
2. (i)-(S),(ii)-(P), (iii)-(Q), (iv)-(R)
3. (i)-(P),(ii)-(Q), (iii)-(S), (iv)-(R)
4. (i)-(Q),(ii)-(P), (iii)-(S), (iv)-(R)
Subtopic: Tools: Vectors: I |
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Match Column-I with column II and select the correct option
1. (a)-(i), (b)-(ii), (c)-(iii), (d)-(iv)
2. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
3. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
| Column-I | Column-II | ||
| (a) | DNA fragmentation | (i) | ssDNA gets embedded in nylon membrane |
| (b) | Electrophoresis | (ii) | Sample DNA and probe DNA from double stranded DNA |
| (c) | Southern blotting | (iii) | DNA fragmentation move to positive pole |
| (d) | Hybridization | (iv) | Fragments of DNA with variable length are formed |
2. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
3. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
Subtopic: Tools |
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Match Column-I with column II and select the correct option
1. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
2. (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
3. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
4. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
| Column-I | Column-II | ||
| (a) | Gel electrophosis | (i) | To combine genes from two organisms |
| (b) | cDNA | (ii) | To make a copy of DNA from mRNA |
| (c) | Genetic Engineering | (iii) | Separate the DNA fragments |
| (d) | PCR | (iv) | Gene multiplication in vitro |
2. (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
3. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
4. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
Subtopic: Tools |
78%
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Match the process of PCR in column I with the corresponding temperature in column II and select the correct option
1. (a)-(ii), (b)-(i), (c)-(iii)
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
| Column-I | Column-II | ||
| (a) | Heat denaturation | (i) | 70-75°C |
| (b) | Annealing | (ii) | 90-98°C |
| (c) | Primer extension | (iii) | 40-60°C |
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
Subtopic: Polymerase Chain Reaction: PCR |
66%
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Identify the process in Column-I with the term in column II and select the correct option
1. (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
2. (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii)
3. (a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
| Column-I | Column-II | ||
| (a) | Amplifications of gene of interest | (i) | Downstream processing |
| (b) | Large scale production of plants in short duration | (ii) | Polymerase chain reaction |
| (c) | Separation and purification of desired product | (iii) | Transformation |
| (d) | Insertion of rDNA into host | (iv) | Micro propagation |
2. (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii)
3. (a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
Subtopic: Bioreactors/Downstream Processing |
71%
From NCERT
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Match the restriction enzyme given in Column-I with their source given in column II and choose the correct option
1. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
2. (a)-(iii), (b)-(i), (c)-(iv), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
| Column-I | Column-II | ||
| (a) | Alu I | (i) | Bacillus amyloliquefaciens H |
| (b) | Bam HI | (ii) | H. influenza Rd. |
| (c) | Eco RI | (iii) | Arthobacter luteus |
| (d) | Hind RI | (iv) | Escherichia coli Ry 13 |
2. (a)-(iii), (b)-(i), (c)-(iv), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
Subtopic: Tools: Vectors: I |
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