Column I | Column II | ||
(a) | Gel electrophoresis | (i) | Chimeric DNA |
(b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
(c) | rDNA technology | (iii) | Palindrome |
(d) | PCR | (iv) | Separation of DNA fragments. |
Column-I | Column-II | ||
i. | Alu I | a. | Bacillus amyloliquefaciens H |
ii. | Bam HI | b. | H. influenza RD |
iii. | Eco RI | c. | Arthobacter luteus |
iv. | Hind II | d. | Escherichia coli Ry 13 |
Column I (Restriction Enzyme) |
Column II (Recognition sequence) |
||
i. | AluI | P. | \(5'---G- \downarrow-A-T-C-C---3'\\3'---C-C-T-A-G-\uparrow-G---5'\) |
ii, | BamHI | Q. | \(5'---A-G-\downarrow-C-T---3'\\ 3' ---T-C- \uparrow -G-A---5' \) |
iii. | EcoRI | R. | \(5'--G-T-C- \downarrow - G-A-C--3' \\ 3'--C-A-G-\uparrow-C-T-G--5'\) |
iv. | HindII | S. | \(5'---G- \downarrow-A-A-T-T-C---3'\\ 3'--- C-T-T-A-A-\uparrow-G---5'\) |
Column-I | Column-II | ||
(a) | DNA fragmentation | (i) | ssDNA gets embedded in nylon membrane |
(b) | Electrophoresis | (ii) | Sample DNA and probe DNA from double stranded DNA |
(c) | Southern blotting | (iii) | DNA fragmentation move to positive pole |
(d) | Hybridization | (iv) | Fragments of DNA with variable length are formed |
Column-I | Column-II | ||
(a) | Gel electrophosis | (i) | To combine genes from two organisms |
(b) | cDNA | (ii) | To make a copy of DNA from mRNA |
(c) | Genetic Engineering | (iii) | Separate the DNA fragments |
(d) | PCR | (iv) | Gene multiplication in vitro |
Column-I | Column-II | ||
(a) | Heat denaturation | (i) | 70-75°C |
(b) | Annealing | (ii) | 90-98°C |
(c) | Primer extension | (iii) | 40-60°C |
Column-I | Column-II | ||
(a) | Amplifications of gene of interest | (i) | Downstream processing |
(b) | Large scale production of plants in short duration | (ii) | Polymerase chain reaction |
(c) | Separation and purification of desired product | (iii) | Transformation |
(d) | Insertion of rDNA into host | (iv) | Micro propagation |
Column-I | Column-II | ||
(a) | Alu I | (i) | Bacillus amyloliquefaciens H |
(b) | Bam HI | (ii) | H. influenza Rd. |
(c) | Eco RI | (iii) | Arthobacter luteus |
(d) | Hind RI | (iv) | Escherichia coli Ry 13 |
Column-I | Column-II | ||
(a) | Heat denaturation | (i) | addition of nucleotides on template |
(b) | Annealing | (ii) | separation of two strands of DNA |
(c) | Primer Extension | (iii) | paring of primer to complementary sequence in DNA |
Column-I | Column-II | ||
(a) | Gel electrophoresis | (i) | Chrimeric DNA |
(b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
(c) | rDNA technology | (iii) | Palindrome |
(d) | PCR | (iv) | Separation of DNA fragments |