Column-I | Column-II | ||
(a) | DNA fragmentation | (i) | ssDNA gets embedded in nylon membrane |
(b) | Electrophoresis | (ii) | Sample DNA and probe DNA from double stranded DNA |
(c) | Southern blotting | (iii) | DNA fragmentation move to positive pole |
(d) | Hybridization | (iv) | Fragments of DNA with variable length are formed |
Column-I | Column-II | ||
(a) | Gel electrophosis | (i) | To combine genes from two organisms |
(b) | cDNA | (ii) | To make a copy of DNA from mRNA |
(c) | Genetic Engineering | (iii) | Separate the DNA fragments |
(d) | PCR | (iv) | Gene multiplication in vitro |
Column-I | Column-II | ||
(a) | Heat denaturation | (i) | 70-75°C |
(b) | Annealing | (ii) | 90-98°C |
(c) | Primer extension | (iii) | 40-60°C |
Column-I | Column-II | ||
(a) | Amplifications of gene of interest | (i) | Downstream processing |
(b) | Large scale production of plants in short duration | (ii) | Polymerase chain reaction |
(c) | Separation and purification of desired product | (iii) | Transformation |
(d) | Insertion of rDNA into host | (iv) | Micro propagation |
Column-I | Column-II | ||
(a) | Alu I | (i) | Bacillus amyloliquefaciens H |
(b) | Bam HI | (ii) | H. influenza Rd. |
(c) | Eco RI | (iii) | Arthobacter luteus |
(d) | Hind RI | (iv) | Escherichia coli Ry 13 |
Column-I | Column-II | ||
(a) | Heat denaturation | (i) | addition of nucleotides on template |
(b) | Annealing | (ii) | separation of two strands of DNA |
(c) | Primer Extension | (iii) | paring of primer to complementary sequence in DNA |
Column-I | Column-II | ||
(a) | Gel electrophoresis | (i) | Chrimeric DNA |
(b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
(c) | rDNA technology | (iii) | Palindrome |
(d) | PCR | (iv) | Separation of DNA fragments |
Assertion (A): | A piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector. |
Reason (R): | The vectors provide the origin of replication. |
1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
2. | (A) is False but (R) is True. |
3. | (A) is True but (R) is False. |
4. | Both (A) and (R) are True and (R) correctly explains (A). |