Electroporation facilitates insertion of foreign DNA into the cell by

1. Changing the porocity of the membrane

2. Changing the electrical potential of the membrane

3. Lysing the cell wall

4. Active transport

The names associated with construction of first Recombinant DNA are

a. Arber, Nathans, Smith

b. Annie Chang, Boyer, Berg, Cohen

c. Howard Temin, Brenner, Sharp

d. Tim Hunt, Hartwell, Nurse

The restriction enzymes most commonly used in rDNA technology are

1. Type 1 Restriction enzymes

2. Type II Restriction enzymes

3. Type III Restriction enzymes

4. Type IV Restriction enzymes

Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because
a. Host DNA is packed into chromosomes
b. Restriction enzymes are ineffective on host DNA
c. Host DNA does not have the restriction site for the Restriction enzymes.
d. Restriction site of host DNA is methylated.

A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?
a. Southern blotting and South Western blotting
b. Northern blotting and western blotting
c. Western blotting and Eastern blotting
d. Southern and Northern blotting

Insertional inactivation of a gene helps in
a. Identification of recombinant clones
b. Identification of deletion mutants
c. Identification of recombinant transformants
d. Elimination of suppression mutants

Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
a. Zinc or tungsten
b. Silicon or gold
c. Tungsten or gold
d. Selenium or Platinum

Insertional inactivation of the lac Z gene forms-

A reporter enzyme used in biotechnology is-

Downstream processing does not include