Q. No.Insertional inactivation of a gene helps in
a. Identification of recombinant clones
b. Identification of deletion mutants
c. Identification of recombinant transformants
d. Elimination of suppression mutants
a. Identification of recombinant clones
b. Identification of deletion mutants
c. Identification of recombinant transformants
d. Elimination of suppression mutants
Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
a. Zinc or tungsten
b. Silicon or gold
c. Tungsten or gold
d. Selenium or Platinum
Insertional inactivation of the lac Z gene forms-
(1) Blue recombinant colonies
(2) Colourless recombinant colonies
(3) Fluorescent green colonies
(4) There is no relation between the lac Z gene and colour of the colony.
A reporter enzyme used in biotechnology is-
(1) Green Fluorescent Protein
(2) Luciferase
(3) b Galactosidase
(4) All of the above
Downstream processing does not include
(1) Quality control
(2) Clinical trials
(3) Product purification
(4) Sampling from bioreactor
Which is not a component of the vector pBR322?
(1) Chloramphenicol selectable marker
(2) Replication proteins
(3) Origin of replication
(4) Cloning site for BamHI enzyme
Two bacteria found to be very useful in genetic engineering are
(1) Diplococcus and Streptomyces
(2) Rhizobium and Xanthomonas
(3) Vibrio and Escherischia
(4) Escherischia and Agrobacterium
Which of the following would you choose as the safest and least cumbersome selectable marker-
(1) Ampicillin resistance gene
(2) Tetracyclin resistance gene
(3) Kanamycin resistance gene
(4) b Galactosidase gene
Recombinant DNA is formed in nature during-
(1) Crossing over at pachytene
(2) Integration of plasmid to form episome
(3) Integration of phage DNA to form prophage
(4) More than one is correct.
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