Q. No.Insertional inactivation of the lac Z gene forms-
(1) Blue recombinant colonies
(2) Colourless recombinant colonies
(3) Fluorescent green colonies
(4) There is no relation between the lac Z gene and colour of the colony.
(1) Blue recombinant colonies
(2) Colourless recombinant colonies
(3) Fluorescent green colonies
(4) There is no relation between the lac Z gene and colour of the colony.
A reporter enzyme used in biotechnology is-
(1) Green Fluorescent Protein
(2) Luciferase
(3) b Galactosidase
(4) All of the above
Downstream processing does not include
(1) Quality control
(2) Clinical trials
(3) Product purification
(4) Sampling from bioreactor
Which is not a component of the vector pBR322?
(1) Chloramphenicol selectable marker
(2) Replication proteins
(3) Origin of replication
(4) Cloning site for BamHI enzyme
Two bacteria found to be very useful in genetic engineering are
(1) Diplococcus and Streptomyces
(2) Rhizobium and Xanthomonas
(3) Vibrio and Escherischia
(4) Escherischia and Agrobacterium
Which of the following would you choose as the safest and least cumbersome selectable marker-
(1) Ampicillin resistance gene
(2) Tetracyclin resistance gene
(3) Kanamycin resistance gene
(4) b Galactosidase gene
Recombinant DNA is formed in nature during-
(1) Crossing over at pachytene
(2) Integration of plasmid to form episome
(3) Integration of phage DNA to form prophage
(4) More than one is correct.
Southern Blotting essentially makes use of a
(1) Buffer
(2) Electrodes
(3) Agarose gel
(4) All of the above
The native charge on DNA molecules is
(1) Positive
(2) Negative
(3) Neutral
(4) DNA is an ampholyte
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