Pvu I restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline Resistance gene

3.      Ampicillin Resistance gene

4.     Ori

Subtopic:  Tools: Vectors: II | Tools |
 81%
From NCERT
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If you have a fungal cell and want to extract DNA, what will be the first enzyme to be used?

1.      Chitinase

2.      Lysozyme

3.      Cellulase

4.     Protease

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 94%
From NCERT
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If a researcher starts his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

1.      Cellulase

2.      Protease

3.      Ribonuclease

4.     Chitinase

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 93%
From NCERT
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If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

1.      Cellulase

2.      Chitinase

3.      Lysozyme

4.     Protease

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 86%
From NCERT
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If a researcher has to use a bacterial cell to isolate DNA from bacterial cells then to remove another nucleic acid, which enzyme should be used?

1.      DNAse

2.      Ribonuclease

3.      Protease

4.      Lysozyme

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 54%
From NCERT
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The precipitation of DNA out of the solution is done by adding

1.      Organic Acid

2.      HCl

3.      Ethanol

4.     Ketone

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 96%
From NCERT
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The end result of precipitation of DNA in suspension is seen

1.      In the form of dark solution

2.      In the form of effervescence

3.      In the form of fine threads

4.      In the form of bubbles

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 86%
From NCERT
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The precipitated DNA can be removed from the suspension

1.      By Filtration

2.      By giving a heat shock

3.      By spooling

4.      By gel electrophoresis

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 85%
From NCERT
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Primers are not

1.      Small

2.      Biologically synthesized

3.      Oligonucleotides

4.     Complementary to the regions of DNA

Subtopic:  Large Scale Production: I | Large Scale Production |
 66%
From NCERT
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Which of the following doesn't go with PCR?

1.    Thermus aquaticus

2.     1 billion copies

3.     Taq polymerase

4.     Dideoxynucleotides

Subtopic:  Large Scale Production: I | Large Scale Production |
 86%
From NCERT
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