If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

1.      Cellulase

2.      Chitinase

3.      Lysozyme

4.     Protease

If a researcher has to use a bacterial cell to isolate DNA from bacterial cells then to remove another nucleic acid, which enzyme should be used?

1.      DNAse

2.      Ribonuclease

3.      Protease

4.      Lysozyme

The precipitation of DNA out of the solution is done by adding

1.      Organic Acid

2.      HCl

3.      Ethanol

4.     Ketone

The end result of precipitation of DNA in suspension is seen

1.      In the form of dark solution

2.      In the form of effervescence

3.      In the form of fine threads

4.      In the form of bubbles

The precipitated DNA can be removed from the suspension

1.      By Filtration

2.      By giving a heat shock

3.      By spooling

4.      By gel electrophoresis

Primers are not

1.      Small

2.      Biologically synthesized

3.      Oligonucleotides

4.     Complementary to the regions of DNA

Which of the following doesn't go with PCR?

1.    Thermus aquaticus

2.     1 billion copies

3.     Taq polymerase

4.     Dideoxynucleotides

If a strand has the following sequence 5’ATTGCCCTAG 3’what will be the sequence of DNA synthesized from its complementary strand.

1.      5’TAACGGGATC 3’

2.      3’TAACGGGATC 5’

3.      5’ATTGCCCTAG 3’

4.     3’ATTGCCCTAG 5’

If you start with 10 molecules of Double stranded DNA, what will be the number of DNA molecules formed after 10 cycles of PCR?

1.      10240

2.      1024

3.      512

4.     5120

What is the final step of PCR?

1.      Extension

2.      Annealing

3.      Denaturation

4.     Extraction