If a researcher has to use a bacterial cell to isolate DNA from bacterial cells then to remove another nucleic acid, which enzyme should be used?

1.      DNAse

2.      Ribonuclease

3.      Protease

4.      Lysozyme

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
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The precipitation of DNA out of the solution is done by adding

1.      Organic Acid

2.      HCl

3.      Ethanol

4.     Ketone

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
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The end result of precipitation of DNA in suspension is seen

1.      In the form of dark solution

2.      In the form of effervescence

3.      In the form of fine threads

4.      In the form of bubbles

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 86%
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The precipitated DNA can be removed from the suspension

1.      By Filtration

2.      By giving a heat shock

3.      By spooling

4.      By gel electrophoresis

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 85%
From NCERT
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Primers are not

1.      Small

2.      Biologically synthesized

3.      Oligonucleotides

4.     Complementary to the regions of DNA

Subtopic:  Large Scale Production: I | Large Scale Production |
 66%
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Which of the following doesn't go with PCR?

1.    Thermus aquaticus

2.     1 billion copies

3.     Taq polymerase

4.     Dideoxynucleotides

Subtopic:  Large Scale Production: I | Large Scale Production |
 86%
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If a strand has the following sequence 5’ATTGCCCTAG 3’what will be the sequence of DNA synthesized from its complementary strand.

1.      5’TAACGGGATC 3’

2.      3’TAACGGGATC 5’

3.      5’ATTGCCCTAG 3’

4.     3’ATTGCCCTAG 5’

Subtopic:  Large Scale Production: I | Large Scale Production |
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If you start with 10 molecules of Double stranded DNA, what will be the number of DNA molecules formed after 10 cycles of PCR?

1.      10240

2.      1024

3.      512

4.     5120

Subtopic:  Large Scale Production: I | Large Scale Production |
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What is the final step of PCR?

1.      Extension

2.      Annealing

3.      Denaturation

4.     Extraction

Subtopic:  Large Scale Production: I | Large Scale Production |
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After 30 cycles of PCR, how many times DNA will be amplified?

1.      Thousand times

2.      Million times

3.      Billion times

4.     Hundreds times

Subtopic:  Large Scale Production: I | Large Scale Production |
 83%
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