Q. No.Which of the enzymes whose recognition sites are shown here would produce sticky ends compatible with BstAUI (5'-G|GTACC-3')?
1. Bcl I (5'-T|GATCA-3')
2. Sun I (5'-C|GTACG-3')
3. Hind III (5'-A|AGCTT-3')
4. Kpn I (5'-GGTAC|C-3')
In transgenics, the expression of transgene in the target tissue is known by
1. Enhancer
2. Transgene
3. Promoter
4. Reporter
Identify the incorrectly matched pair:
restriction enzyme recognition site
1. EcoRI 5’GAATTC3’
3’CTTAAG5’
2. HindII 5’GTPyPuAC3’
3’CAPuPyTG3’
3. SmaI 5’GGATTC3’
3’CTTAGG5’
4. HindIII 5’AAGCTT3’
3’TTCGAA5’
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Identify the wrong statement with regards to restriction enzyme:
| 1. | They cut the strength at palindromic sites. |
| 2. | They are useful in genetic engineering. |
| 3. | Sticky ends can be joined by using DNA ligases. |
| 4. | Each restriction enzyme functions by inspecting the length of a DNA sequence. |
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Bacteria can protect themselves from getting infected by bacteriophages as they synthesize:
1. Methylase
2. Ligases
3. Plasmids
4. Endonucleases
A fragment of DNA, cut by a restriction enzyme, forms bonds with other DNA molecules that have
1. Been fragmented by the same restriction enzyme
2. Sticky ends
3. Plasmid component
4. Attached methyl group
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If you are using a vector with lacZ as a screenable marker, which of the following bacterial strains would you want to use as host cells?
1. lacO-
2. lacI-
3. lacZ-
4. Any of these would work.
Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?
1. electroporation
2. liposome transfer
3. microinjection
4. particle bombardment
Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactal bumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of rDNA technology.
(D) Disarmed pathogen vectors are also used in transfer of r-DNA into the host.
Which are the two statements having mistakes?
1. Statement (B) and (C)
2. Statement (C) and (D)
3. Statement (A) and (C)
4. Statement (A) and (B)
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‘Competence’ can be induced in a bacterium by treating them with:
1. Polyethylene glycol
2. A specific divalent cation
3. Colchicine
4. Alkaline phosphatase
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