Given below are two statements: One is labeled as Assertion (A) and the other is labeled as Reason (R)
Assertion (A): | Restriction enzyme is a type of endonuclease |
Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA |
In the light of the above statements, choose the correct answer from the options given below:
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): | In rDNA technology non-recombinants transformed bacteria grow on the medium containing ampicillin as well as medium containing tetracycline |
Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Identify the properties of a good vector used in rDNA technology:
(a) | It should have origin of replication supporting a high copy number |
(b) | It should have preferably more than '2' recognition sites |
(c) | The restriction sites in vector should be in the antibiotic- resistant genes |
(d) | It should have suitable marker genes |
(e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
List I | List II |
A. Chloramphenicol | I. Join the cut ends of DNA molecule |
B. Ethidium bromide | II. Makes bacterial cell competent to take up DNA |
C. DNA ligase | III. Selectable marker |
D. Calcium | IV. Agarose gel staining |
List I | List II | ||
(a) | Gene gun | (i) | Replacement of a faulty gene by a normal healthy gene |
(b) | Gene therapy | (ii) | Used for transfer of gene |
(c) | Gene cloning | (iii) | Total DNA in the cells of an organism |
(d) | Genome | (iv) | To obtain identical copies of a particular DNA molecule |
(a) | (b) | (c) | (d) | |
1. | (ii) | (i) | (iv) | (iii) |
2. | (i) | (iii) | (ii) | (iv) |
3. | (iv) | (i) | (iii) | (ii) |
4. | (ii) | (iii) | (iv) | (i) |
(a) | Agarose is a natural polymer obtained from sea-weed. |
(b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
(c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
(d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
1. | (a) and (b) only | 2. | (a), (b) and (c) only |
3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
a. | They float freely in the cytoplasm of certain bacterial cells. |
b. | They have the ability to replicate, independent of the genomic DNA. |
c. | Herbert Boyer developed a method of removing and reinserting them in the cells. |
d. | They are circular ssDNA molecules. |