Q. No.1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension
The DNA fragments separated on an agarose gel can be visualized after staining with:
1. Acetocarmine
2. Aniline blue
3. Ethidium bromide
4. Bromophenol blue
The process of separation and purification of expressed protein before marketing is called:
1. Downstream processing
2. Bioprocessing
3. Post-product processing
4. Upstream processing
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
A gene whose expression helps to identify transformed cell is known as:
| 1. | Vector | 2. | Plasmid |
| 3. | Structural gene | 4. | Selectable marker |
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
| 1. | The smaller the fragment size, the farther it moves |
| 2. | Positively charged fragments move to farther end |
| 3. | Negatively charged fragments do not move |
| 4. | The larger the fragment size, the farther it moves |
Which of the following is not a component of downstream processing?
1. Separation
2. Purification
3. Preservation
4. Expression
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
1. Eco Rl
2. taq polymerase
3. polymerase lll
4. ligase
Which of the following restriction enzymes produces blunt ends?
1. Sal l
2. Eco RV
3. Xho
4. Hind lll
2. DNase I
3. RNase
4. Hind II
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