- 1(current)
Q. No.What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes |
| II: | Inability of inclusion of desirable traits from other species |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
| 1. | DNA can be seen in visible light |
| 2. | DNA can be seen without staining in visible light |
| 3. | Ethidium bromide stained DNA can be seen in visible light |
| 4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3. Beta Galactosidase
4. Chloramphenicol acetyl transferase
A donor DNA and plasmid vector DNA of E. coli are cut by EcoRI. The plasmid contains genes for resistance to ampicillin and tetracycline. The ECoRI recognition sequence lies within the gene for tetracycline resistance. Ligase is used, and the recombinant DNA is produced. The plasmids are transferred to E.coli with the help of electroporation. Samples of the bacterial colonies are then grown in four different media: nutrient medium plus ampicillin, nutrient medium plus tetracycline, nutrient medium plus ampicillin and tetracycline, and nutrient medium without antibiotics. Non-recombinant transformants will grow on:
| 1. | the nutrient medium plus ampicillin, but not on the medium containing tetracycline. |
| 2. | only on the medium containing both antibiotics. |
| 3. | on the medium containing tetracycline, but not on the medium containing ampicillin. |
| 4. | on all four types of mediums. |
Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
| 1. | Recombinant protein | 2. | Native protein |
| 3. | Pro-protein | 4. | Exotic protein |
Identify (a) and (b) bioreactors respectively in the given diagram:
1. Simple stirred-tank and sparged stirred – tank
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift
- 1(current)
Q. No.
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