1. | they are circular |
2. | they replicate autonomously |
3. | they are extra-chromosomal DNA molecules found in many bacterial cells |
4. | they carry genes vital for normal survival and reproduction |
I: | Ori controls the copy number of DNA per cell |
II: | Multiple cloning sites are recognition sequences of common restriction enzymes |
III: | Genes for antibiotic resistance are used as selectable markers |
1. | Only I and II are correct |
2. | Only I and III are correct |
3. | Only II and III are correct |
4. | I, II, and III are correct |
1. | The process of separation and purification of expressed protein before marketing is called as downstream processing |
2. | DNA precipitation out of a mixture of biomolecules can be achieved by treatment with a divalent cation such as calcium ion |
3. | Stirred-tank bioreactors have been designed for availability of oxygen throughout the process |
4. | Reporter genes can be used as markers for selecting successfully transformed cells |
1. | Microinjection | 2. | Ti Plasmid |
3. | Retroviral vectors | 4. | Liposomes |
I: | is used to synthesize multiple copies of the gene [or DNA] in vitro. |
II: | uses a thermostable DNA dependent DNA polymerase. |
III: | is now routinely used to detect HIV in suspected AIDS patient. |
IV: | is being used to detect mutations in genes in suspected cancer patients. |
1. | All except I are true | 2. | Only II and III are true |
3. | Only IV is not true | 4. | I, II, III and IV are true |
I: | The gene that was not inactivated helps in selecting the transformants. |
II: | The gene that was insertionally inactivated helps in selection of recombinants. |
1. | Only I is correct |
2. | Only II is correct |
3. | Both I and II are correct |
4. | I, II and III are correct |
I: | The used medium is drained out from one side while the fresh medium is added from the other. |
II: | The cells are maintained in their physiologically most active log/exponential phase. |
III: | A larger biomass is produced leading to higher yields of desired proteins. |
1. | Only I and II are correct |
2. | Only I and III are correct |
3. | Only II and III are correct |
4. | I, II and III are correct |
1. | EcoR I | 2. | Hind II |
3. | Bam HI | 4. | Sma I |
I: | This stage is after the biosynthetic stage. |
II: | The processes include separation and purification. |
1. | Only I | 2. | Only II |
3. | Both I and II | 4. | Neither I nor II |
Assertion (A): | When constructing rDNA, the vector DNA should never be cut by the same restriction endonuclease that was used to cut the donor DNA |
Reason (R): | Use of same restriction endonuclease will generate same kind of sticky ends retarding the function of DNA ligase. |
1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
2. | Both (A) and (R) are True but (R) is not the correct explanation of (A) |
3. | (A) is True but (R) is False |
4. | Both (A) and (R) are False |