Using reverse transcriptase or gene synthesis has an advantage over the shotgun approach in obtaining a copy of the gene required as:
1. | the gene produced can easily be ligated to the vector |
2. | the gene produced is not a split gene |
3. | the gene produced does not require a promoter to be expressed |
4. | the gene produced does not require an ori to replicate |
The promoter site and the terminator site for transcription are located at:
1. | 3' (downstream) end and 5' (upstream) end, respectively of the transcription unit |
2. | 5' (upstream) end and 3' (downstream) end, respectively of the transcription unit |
3. | the 5' (upstream) end |
4. | the 3' (downstream) end |
With regard to mature mRNA in eukaryotes:
1. | exons and introns do not appear in the mature RNA. |
2. | exons appear but introns do not appear in the mature RNA. |
3. | introns appear but exons do not appear in the mature RNA. |
4. | both exons and introns appear in the mature RNA. |
Discontinuous synthesis of DNA occurs in one strand, because:
1. | DNA molecule being synthesised is very long |
2. | DNA-dependent DNA polymerase catalyses polymerisation only in one direction (5’ → 3’) |
3. | it is a more efficient process |
4. | DNA ligase joins the short stretches of DNA |
Which of the following statements is correct about the role of regulatory proteins in transcription in prokaryotes?
1. | They only increase the expression |
2. | They only decrease the expression |
3. | They interact with RNA polymerase but do not affect the expression |
4. | They can act both as activators and as repressors |
While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine = 29%, Guanine = 17%, Cytosine = 32%, Thymine = 17%. Considering Chargaff’s rule it can be concluded that:
1. | it is a double-stranded circular DNA |
2. | It is single-stranded DNA |
3. | It is a double-stranded linear DNA |
4. | No conclusion can be drawn |
DNA fingerprinting is related to:
1. | Molecular analysis of profiles of DNA samples |
2. | Analysis of DNA samples using imprinting devices |
3. | Techniques used for molecular analysis of different specimens of DNA |
4. | Techniques used in the identification of fingerprints of different persons |
A nutritionally wild-type organism that does not require additional biochemicals is:
1. | Prototroph | 2. | Auxotroph |
3. | Phenotype | 4. | Autotroph |
What is it that forms the basis of DNA Fingerprinting?
1. | The relative proportions of purines and pyrimidines in DNA. |
2. | The relative difference in the DNA occurrence in blood, skin, and saliva. |
3. | The relative amount of DNA in the ridges and grooves of the fingerprints. |
4. | Satellite DNA occurring as highly repeated short DNA segments |
Select the correct option:
The direction of RNA synthesis |
The direction of reading of the template DNA strand |
|
1. |
5' - 3' |
3' - 5' |
2. |
3' - 5' |
5' - 3' |
3. |
5' - 3' |
5' - 3' |
4. |
3' - 5' |
3' - 5' |