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Q. No.

The primary RNA transcript in eukaryotes contains introns. This can be demonstrated by:

I: A comparison of the DNA and mRNA sequences.
II: A comparison of the genomic DNA and cDNA sequences.
III: A hybridization between DNA and mRNA molecules.
 
1. I and II only 2. I and III only
3. II and III only 4. I, II, and III

Subtopic:  Transcription: I |
 54%
Level 3: 35%-60%
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What would be true for the mutation that leads to sickle cell anemia ?

I: Is a base substitution.
II: Is a deleterious mutation.
III: Is a missense mutation.
 
1. I and II only 2. I and III only
3. II and III only 4. I, II, and III
Subtopic:  Mutations and Genetic Code |
Level 3: 35%-60%
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Expression of the lac operon was completely repressed. This will lead to:

1. The low level of lacZ, lacY, and lacA gene expression will continue.
2. Allolactose would accumulate within the cell and become toxic.
3. Lactose would not be converted into the inducer and the operon could not be induced.
4. The cell will not be able to use glucose as well.
Subtopic:  Gene Regulation: Lac Operon |
 58%
Level 3: 35%-60%
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What type of changes will lead to a frameshift mutation?

I: A transition II: A transversion III: A deletion of 3 bp
 
1. I and II only 2. III only
3. I, II, and III 4. None

Subtopic:  Mutations and Genetic Code |
Level 3: 35%-60%
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Identify the correct statements regarding mutations:

I: Are permanent changes in the DNA sequence or structure.
II: Produce allelic variation.
III: Are more likely to be harmful than beneficial.
 
1. I and II only 2. I and III only
3. II and III only 4. I, II, and III
Subtopic:  Mutations and Genetic Code |
 74%
Level 2: 60%+
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Regulation of gene expression very commonly involves transcriptional regulation. The most important significance of this is that it:

1. is highly efficient at completely preventing transcription.
2. allows the cell to only produce proteins that are needed at the time.
3. can be induced by a repressor protein.
4. can be done only in prokaryotes.
Subtopic:  Gene Regulation in Eukaryotes |
 59%
Level 3: 35%-60%
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What would be the actual inducer for the lac operon?

1.lactose2.allolactose
3.ß-galactosidase4.galactose

Subtopic:  Gene Regulation: Lac Operon |
Level 3: 35%-60%
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The origin of replication is a particular sequence in a genome at which replication is initiated. This region is AT-rich because:

1. DnaA proteins bind to AT-rich sites.
2. DnaC proteins bind to AT-rich sites.
3. AT base pairs are more easily separated than GC base pairs.
4. AT base pairs contain more hydrogen bonds than GC base pairs.

Subtopic:  DNA Replication |
 77%
Level 2: 60%+
Hints

Identify the correct statements regarding a replisome:

I: It is a complex of several proteins.
II: It includes enzymes like DNA polymerase, DNA helicase, and primase.
III: It is found at the replication fork during DNA synthesis.
 
1. I and II only 2. I and III only
3. II and III only 4. I, II, and III
Subtopic:  DNA Replication |
 73%
Level 2: 60%+
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T2 bacteriophage was used by Hershey and Chase to conclusively prove that DNA and not protein is the genetic material because:

1. It makes capsomeres with the help of its genes.
2. It injects its genetic material into a bacterial cell.
3. It can undergo either the lytic or lysogenic life cycle
4. It enters the bacterial cell to cause infection.
Subtopic:  Search for Genetic Material |
 73%
Level 2: 60%+
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