During translation initiation in prokaryotes, a GTP molecule is needed in:
| 1. | Formation of formyl-met-tRNA |
| 2. | Binding of 30S subunit of the ribosome with mRNA |
| 3. | Association of 30 S-mRNA with formyl met-tRNA |
| 4. | Association of 50 S subunit of the ribosome with the initiation complex |
Identify the correct statements from the given statements and choose the correct option:
| I: | Both prokaryotic and eukaryotic mRNAs have a 5' cap. |
| II: | Only prokaryotic mRNAs are polyadenylated at the 3' end. |
| III: | In prokaryotes, transcription is coupled to translation. |
| IV: | In eukaryotes, RNA splicing occurs after the mRNA is transported into the cytoplasm. |
| V: | RNA splicing requires the formation of a spliceosome. |
| VI: | Both prokaryotic and eukaryotic mRNAs are synthesized by RNA polymerase. |
| 1. | III, V, and VI only |
| 2. | II, III, V, and VI only |
| 3. | I, III, V, and VI only |
| 4. | I, III, IV, V, and VI only |
With regard to mature mRNA in eukaryotes:
| 1. | exons and introns do not appear in the mature RNA. |
| 2. | exons appear but introns do not appear in the mature RNA. |
| 3. | introns appear but exons do not appear in the mature RNA. |
| 4. | both exons and introns appear in the mature RNA. |