Transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization is called as:
1. | DNA profiling | 2. | PCR |
3. | Southern blotting | 4. | Elution |
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. | Transform bacteria with recombinant DNA molecule. |
II. | Cut the plasmid DNA using restriction enzymes. |
III. | Extract plasmid DNA from bacterial cells. |
IV. | Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. |
V. | Use ligase to seal plasmid DNA to non-plasmid DNA. |
1. I, II, IV, III, V
2. II, III, V, IV, I
3. III, II, IV, V, I
4. III, IV, V, I, II
The "Southern" technique involves:
1. | the detection of DNA fragments on membranes by specific radioactive antibodies. |
2. | the detection of proteins on membranes using a radioactive DNA probe. |
3. | the detection of DNA fragments on membranes by a radioactive DNA probe. |
4. | the detection of proteins on membranes using specific radioactive antibodies. |
A piece of nucleic acid used to find a gene, by forming a hybrid with it, is called a
1. Probe
2. Vector
3. Restriction sequence
4. Retrovirus
The size of VNTR [used in DNA fingerprinting] varies from:
1. | 0.1 to 20 kb | 2. | 20 to 30 kb |
3. | 50 to 100 kb | 4. | 115 to 215 kb |
What is it that forms the basis of DNA Fingerprinting?
1. | The relative difference in the DNA occurrence in blood, skin and saliva |
2. | The relative amount of DNA in the ridges and grooves of the fingerprints |
3. | Satellite DNA occurs as highly repeated short DNA segments |
4. | The relative proportions of purines and pyrimidines in DNA |