What would be true for both Eukaryotic and Prokaryotic gene expression?

1.  Coupling of transcription and translation
2.  Post-transcriptional removal of introns
3.  Binding of RNA polymerase to the promoter to initiate transcription
4.  The eukaryotic hnRNA and prokaryotic RNA are polycistronic
Subtopic:  Transcription: I |
 75%
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Which of the following statements is correct about the role of regulatory proteins in transcription in prokaryotes?

1. They only increase the expression
2. They only decrease the expression
3. They interact with RNA polymerase but do not affect the expression
4. They can act both as activators and as repressors
Subtopic:  Gene Regulation: Lac Operon |
 69%
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What is it that forms the basis of DNA Fingerprinting?

1. The relative proportions of purines and pyrimidines in DNA.
2. The relative difference in the DNA occurrence in blood, skin, and saliva.
3. The relative amount of DNA in the ridges and grooves of the fingerprints.
4. Satellite DNA occurring as highly repeated short DNA segments
Subtopic:  DNA Fingerprinting |
 86%
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DNA fingerprinting is related to:

1. Molecular analysis of profiles of DNA samples
2. Analysis of DNA samples using imprinting devices
3. Techniques used for molecular analysis of different specimens of DNA
4. Techniques used in the identification of fingerprints of different persons
Subtopic:  DNA Fingerprinting |
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What would be the actual inducer for the lac operon?

1.lactose2.allolactose
3.ß-galactosidase4.galactose

Subtopic:  Gene Regulation: Lac Operon |
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The primary RNA transcript in eukaryotes contains introns. This can be demonstrated by:

I: A comparison of the DNA and mRNA sequences.
II: A comparison of the genomic DNA and cDNA sequences.
III: A hybridization between DNA and mRNA molecules.
 
1. I and II only 2. I and III only
3. II and III only 4. I, II, and III
Subtopic:  Transcription: I |
 54%
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Discontinuous synthesis of DNA occurs in one strand, because:

1. DNA molecule being synthesised is very long
2. DNA-dependent DNA polymerase catalyses polymerisation only in one direction (5’ → 3’)
3. it is a more efficient process
4. DNA ligase joins the short stretches of DNA

Subtopic:  The DNA | DNA Replication: I | DNA Replication: II | DNA Replication:III |
 89%
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Match each item in Column I with one item in Column II and chose your answer from the codes given below:

Column I

(Scientist)

Column II

(Contribution)

I. Hargobind Khorana

II. Norman Borlaug

III. Cohen and Boyer

IV. Ian Wilmut

1. Green revolution

2. First man-made gene

3. First cloned mammal

4. rDNA technology

 
Codes: I II III IV
1. 1 2 3 4
2. 2 1 4 3
3. 2 1 3 4
4. 1 2 4 3
Subtopic:  Genetic Code: I |
 82%
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Using reverse transcriptase or gene synthesis has an advantage over the shotgun approach in obtaining a copy of the gene required as:

1. the gene produced can easily be ligated to the vector
2. the gene produced is not a split gene
3. the gene produced does not require a promoter to be expressed
4. the gene produced does not require an ori to replicate

Subtopic:  DNA Replication |
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Heat-killed S form Streptococcus extract treated with proteases is mixed with live R form Streptococcus cells and injected into living mice. What is most likely to happen?

1. The mice would live.
2. The mice would die.
3. The mice's blood would have dead R cells.
4. A new strain of Streptococcus will be present in mice.
Subtopic:  Search for Genetic Material:I | Search for Genetic Material: II | Search for Genetic Material |
 85%
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