Identify the incorrectly matched pair:

   restriction enzyme                   recognition site

    1. EcoRI                                5’GAATTC3’

                                                3’CTTAAG5’

     2. HindII                               5’GTPyPuAC3’

                                                3’CAPuPyTG3’

     3. SmaI                                5’GGATTC3’

                                                3’CTTAGG5’

    4. HindIII                              5’AAGCTT3’

                                                3’TTCGAA5’

Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactal bumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of rDNA technology.
(D) Disarmed pathogen vectors are also used in transfer of r-DNA into the host.
Which are the two statements having mistakes?
1. Statement (B) and (C)
2. Statement (C) and (D)
3. Statement (A) and (C)
4. Statement (A) and (B)

The introduction of t-DNA into plants involves:
1. Altering the pH of the soil, then heat shocking the plants
2. Exposing the plants to cold for a brief period
3. Allowing the plant roots to stand in water
4. Infection of the plant by Agrobacterium tumefaciens

Ti-plasmid is used for making transgenic plants. It is obtained from
1. Azotobacter
2. Agrobacterium
3. Rhizobium in leguminous root
4. Yeast

In transgenics, the expression of transgene in the target tissue is known by
1. Enhancer
2. Transgene
3. Promoter
4. Reporter

The linking of antibiotic resistance gene with the plasmid vector became possible due to       
1. DNA polymerase
2. Exonucleases
3. DNA ligase
4. Endonucleases

Given below is a sample of a portion of DNA strand. What is so special shown in it
5' GAATTC 3'
3' CTTAAG 5'
1. Replication completed
2. Deletion mutation
3. Start codon at the 5' end
4. Palindromic sequence of base pairs

There is a restriction endonuclease called EcoRI. What does ‘co’ part in it stand for?
1. Colon
2. Coelom
3. Coenzyme
4. coli

Agarose extracted from sea weeds finds use in
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis

Which one of the following techniques made it possible to genetically engineer living organisms?
1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization