A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites

Subtopic:  Cloning Vector |
 56%
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Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?

1.endonuclease2.exonuclease
3.DNA ligase4.Hind – II

Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II |
 87%
From NCERT
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The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:

1.  Transduction 2.  Conjugation
3.  Transformation 4.  Translation

Subtopic:  Host & Desired DNA: I | Host & Desired DNA: II |
 52%
From NCERT
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

1.Charge only2.Size only
3.Charge-to-size ratio4.All of the above

Subtopic:  Selectable Markers: I | Selectable Markers: II | Host & Desired DNA | Tools |
 68%
From NCERT
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An antibiotic resistance gene in a vector usually helps in the selection of:

1. Competent bacterial cells

2. Transformed bacterial cells

3. Recombinant bacterial cells

4. None of the above

Subtopic:  Selection of Recombinant Transformants |
 51%
From NCERT
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The significance of the 'heat shock' method in bacterial transformation is to facilitate:

1.  Binding of DNA to the cell wall
2.  Uptake of DNA through membrane transport proteins
3.  Uptake of DNA through transient pores in the bacterial cell wall
4.  Expression of antibiotic resistance gene

Subtopic:  Host & Desired DNA: I | Host & Desired DNA: II |
 82%
From NCERT
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A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:

1.  Human genes may have intron which bacteria cannot process
2.  Amino acid codons for humans and bacteria are different
3.  Human protein is formed but degraded by bacteria
4.  All of the above

Subtopic:  Tools | General Design of an rDNA experiment |
 61%
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Who among the following was awarded the Nobel Prize for the development of the PCR technique?

1.Herbert Boyer2.Hargovind Khurana
3.Kary Mullis4.Arthur Kornberg

Subtopic:  Polymerase Chain Reaction: PCR |
 71%
From NCERT
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Which of the following cannot be used as a vector in rDNA technology?

1.Plasmid2.Phage DNA
3.Bacterium4.YAC

Subtopic:  Tools |
 59%
From NCERT
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Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?

1.  Plastid  2.  Bacteriophages
3.  BAC 4.   Plasmid
Subtopic:  Cloning Vector |
 66%
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