Who among the following was awarded the Nobel Prize for the development of the PCR technique?
1. | Herbert Boyer | 2. | Hargovind Khurana |
3. | Kary Mullis | 4. | Arthur Kornberg |
Which of the following cannot be used as a vector in rDNA technology?
1. | Plasmid | 2. | Phage DNA |
3. | Bacterium | 4. | YAC |
Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. | Plastid | 2. | Bacteriophages |
3. | BAC | 4. | Plasmid |
It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:
1. | it allows a cell to recognize fragments produced by the enzyme |
2. | the single-stranded ends serve as starting points for DNA replication |
3. | the fragments will bond to other fragments with complementary single-stranded ends |
4. | only single-stranded DNA segments can code for proteins |
Restriction enzymes do not act on the DNA of the host cell in which they are produced, because :
1. | Host DNA is packed into chromosomes |
2. | Restriction enzymes are ineffective on host DNA |
3. | Host DNA does not have the restriction site for the restriction enzymes. |
4. | Restriction site of host DNA is methylated. |
Look at the given hypothetical vector-
Suppose we cut the desired gene as well as the plasmid with the help of the Restriction enzyme EcoR I and ligate them. What would be true?
1. | Kanamycin resistance will select the transformants from non-transformants while tetracycline resistance will select recombinant transformants from non-recombinant transformants. |
2. | Tetracyclin resistance will select the transformants from non-transformants while kanamycin resistance will select recombinant transformants from non-recombinant transformants. |
3. | Both will select only transformants. |
4. | Both will select only recombinants. |
A piece of DNA is inserted within the gene of beta-galactosidase in the E.coli plasmid. X-gal is added to the medium during screening. The colonies of recombinant transformants:
1. | will show an increase in growth |
2. | will not be able to survive |
3. | will give a deep blue color |
4. | will not produce any color |
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:
1. | Human genes may have intron which bacteria cannot process |
2. | Amino acid codons for humans and bacteria are different |
3. | Human protein is formed but degraded by bacteria |
4. | All of the above |
The significance of the 'heat shock' method in bacterial transformation is to facilitate:
1. | Binding of DNA to the cell wall |
2. | Uptake of DNA through membrane transport proteins |
3. | Uptake of DNA through transient pores in the bacterial cell wall |
4. | Expression of antibiotic resistance gene |
An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above