Which one of the following is used as a vector for cloning genes into higher organisms?
1. Retrovirus
2. Baculovirus
3. Salmonella typhimurium
4. Rhizopusnigricans
Restriction endonucleases are enzymes which
1. Remove nucleotides from the ends of the DNA molecule.
2. Make cuts at specific positions within the DNA molecule.
3. Recognize a specific nucleotide sequence for binding of DNA ligase.
4. Restrict the action of the enzyme DNA polymerase.
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1. 5’______CACGTA_______3’
3’______CTVAGT_______5’
2. 5’______CGTTCG_______3’
3’______ATGGTA_______5’
3. 5’______GATATG_______3’
3’______CTACTA_______5’
4. 5’______GAATTC_______3’
3’______CTTAAG_______5’
Which of the following are used in gene cloning?
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleotides
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosomes
2. Yeast artificial chromosomes
3. Plasmid
4. Cosmid
The cutting of DNA at specific locations became possible with the discovery of
1. Ligases
2. Restriction enzymes
3. Probes
4. Selectable markers
Which of the following is not a feature of the plasmids?
1. Independent replication
2. Circular structure
3. Transferable
4. Single-stranded
DNA fragments are
1. Negatively charged
2. Neutral
3. Either positively or negatively charged depending on their size.
4. Positively charged.
Match the following enzymes with their functions:
Column-I |
Column-II |
||
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
Options: | (a) | (b) | (c) | (d) |
1. | (iii) | (i) | (iv) | (ii) |
2. | (iii) | (iv) | (i) | (ii) |
3. | (iv) | (iii) | (i) | (ii) |
4. | (ii) | (iv) | (i) | (iii) |
Polyethylene glycol method is used for
1. Energy production from sewage.
2. Gene transfer without a vector
3. Biodiesel production.
4. Seedless fruit production.