What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
I: | Inclusion and multiplication of undesirable genes |
II: | Inability of inclusion of desirable traits from other species |
1. | Only I | 2. | Only II |
3. | Both I and II | 4. | Neither I nor II |
A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
1. | It includes the centromere |
2. | It becomes a part of chromosome |
3. | It does not contain any intron sequences |
4. | It is a single stranded polynucleotide |
The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
1. | Banting and Best | 2. | Bolivar and Rodriguez |
3. | Hershey and Chase | 4. | Cohen and Boyer |
The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:
1. | Salmonella typhimurium | 2. | Escherechia coli |
3. | Agrobacterium tumefaciens | 4. | Haemophilus influenzae |
The ability of plasmids and bacteriophage DNA to replicate within bacterial cells:
1. | depends on a particular or sequence of the main chromosomal DNA |
2. | is possible due to promoter sequence present in the insert |
3. | cannot occur without telomeric sequences |
4. | is independent of the control of chromosomal DNA |
What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
1. | Ori | 2. | rop |
3. | Selectable markers | 4. | Promoter in the insert |
In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:
1. | 1 and 2 | 2. | 3 and 4 |
3. | 6 and 7 | 4. | 5 and 8 |
In agarose gel electrophoresis, the DNA fragments produced by restriction enzyme digestion:
1. | will move towards anode with smaller fragments moving farther than the larger segments |
2. | will move towards anode with larger fragments moving farther than the smaller segments |
3. | will move towards cathode with smaller fragments moving farther than the larger segments |
4. | will move towards cathode with larger fragments moving farther than the smaller segments |
If you want to isolate DNA in pure form from a fungal cell, the first enzyme required normally would be:
1. | Restriction endonuclease | 2. | DNA ligase |
3. | DNA polymerase | 4. | Chitinase |
A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3. Beta Galactosidase
4. Chloramphenicol acetyl transferase