Consider the two statements:
I: | It is desirable to use restriction enzymes produce 'sticky ends' for cloning experiments in genetic engineering. |
II: | If the donor DNA and the vector DNA are cut by such same restriction endonuclease, ligation of the two is easy. |
1. | Both I and II are correct and II explains I |
2. | Both I and II are correct but II does not explain I |
3. | Only I is correct |
4. | Both I and II are incorrect |
The steps included in a cycle of Polymerase Chain Reaction are:
1. | initiation, elongation, and termination |
2. | transfection, transformation, and transduction |
3. | denaturation, annealing, and extension |
4. | restriction, electrophoresis, and elution |
Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
1. | wheat | 2. | soybean |
3. | rice | 4. | barley |
If you want to isolate DNA in pure form from a fungal cell, the first enzyme required normally would be:
1. | Restriction endonuclease | 2. | DNA ligase |
3. | DNA polymerase | 4. | Chitinase |
In agarose gel electrophoresis, the DNA fragments produced by restriction enzyme digestion:
1. | will move towards anode with smaller fragments moving farther than the larger segments |
2. | will move towards anode with larger fragments moving farther than the smaller segments |
3. | will move towards cathode with smaller fragments moving farther than the larger segments |
4. | will move towards cathode with larger fragments moving farther than the smaller segments |
In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:
1. | 1 and 2 | 2. | 3 and 4 |
3. | 6 and 7 | 4. | 5 and 8 |
What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
1. | Ori | 2. | rop |
3. | Selectable markers | 4. | Promoter in the insert |
The ability of plasmids and bacteriophage DNA to replicate within bacterial cells:
1. | depends on a particular or sequence of the main chromosomal DNA |
2. | is possible due to promoter sequence present in the insert |
3. | cannot occur without telomeric sequences |
4. | is independent of the control of chromosomal DNA |
The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:
1. | Salmonella typhimurium | 2. | Escherechia coli |
3. | Agrobacterium tumefaciens | 4. | Haemophilus influenzae |
The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
1. | Banting and Best | 2. | Bolivar and Rodriguez |
3. | Hershey and Chase | 4. | Cohen and Boyer |