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Q. No.Primers used in PCR are:
1.
a complementary DNA sequence synthesised on the template of hnRNA
2.
artificially synthesised or sequences used to induce replication of DNA
3.
a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome
4.
small chemically synthesised oligonucletides that are complementary to the regions of DNA
Subtopic: Polymerase Chain Reaction: PCR |
72%
Level 2: 60%+
Hints
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
In the light of the above statements, choose the most appropriate answer from the options given below :
| Assertion (A): | It is possible to select a bacterial cell transformed with a recombinant DNA bearing gene for an antibiotic resistance if the transformed cells are spread on agar plates containing the antibiotic. |
| Reason (R): | Due to insertional inactivation of the gene for antibiotic resistance gene, only the untransformed bacterial cells will grow and the transformed bacterial cells will die. |
| 1. | Both (A) and (R) are False. |
| 2. | Both (A) and (R) are True and (R) explains (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True but (R) does not explain (A). |
Subtopic: Cloning Vector |
Level 3: 35%-60%
Hints
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
In the light of the above statements, choose the most appropriate answer from the options given below :
| Assertion (A): | There is a need for the recombinant DNA to be expressed in recombinant DNA technology procedures. |
| Reason (R): | In almost all recombinant DNA technologies, the ultimate aim is to produce a desirable protein. |
| 1. | Both (A) and (R) are False. |
| 2. | Both (A) and (R) are True and (R) explains (A). |
| 3. | Both (A) and (R) are True but (R) does not explain (A). |
| 4. | (A) is True but (R) is False. |
Subtopic: Cloning Vector |
74%
Level 2: 60%+
Hints
Regarding stirred-tank reactor:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | It is usually spherical in shape. |
| II: | The stirrer facilitates even mixing and oxygen availability throughout the reactor. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Bioreactors/Downstream Processing |
66%
Level 2: 60%+
Hints
Elution is:
| 1. | separating the restricted DNA fragments on agarose gel |
| 2. | staining the separated DNA fragments with ethidium bromide |
| 3. | cutting out of the separated bands of DNA from the agarose gel and extracting them from the gel piece |
| 4. | constructing rDNA by joining the purified DNA fragments to the cloning vector |
Subtopic: Transforming Plant & Animal Cell | Obtaining Copy of Gene from Donor DNA |
82%
Level 1: 80%+
Hints
A cloning vector has two antibiotic resistance genes: tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinant transformants would survive on the medium containing:
| 1. | ampicillin but not tetracycline |
| 2. | tetracycline but not ampicillin |
| 3. | both tetracycline and ampicillin |
| 4. | neither tetracycline nor ampicillin |
Subtopic: Cloning Vector |
76%
Level 2: 60%+
Hints
To obtain DNA in pure form, the enzyme that should never be used to hydrolyse biomolecules in a cell lysate, will be:
| 1. | Lysozyme or Chitinase | 2. | DNase |
| 3. | DNAs | 4. | Proteases |
Subtopic: Process of Biotech |
76%
Level 2: 60%+
Hints
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
In the light of the above statements, choose the most appropriate answer from the options given below :
| Assertion (A): | Bioreactors are important in biotechnology procedures to obtain desired products. |
| Reason (R): | Bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions |
| 1. | Both (A) and (R) are False. |
| 2. | Both (A) and (R) are True and (R) explains (A). |
| 3. | Both (A) and (R) are True but (R) does not explain (A). |
| 4. | (A) is True but (R) is False. |
Subtopic: Bioreactors/Downstream Processing |
91%
Level 1: 80%+
Hints
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
In the light of the above statements, choose the most appropriate answer from the options given below :
| Assertion (A): | Agar gel electrophoresis is employed to check the progression of a restriction enzyme digestion. |
| Reason (R): | Restriction enzyme digestion is performed by incubating purified DNA molecules with the restriction enzymes, at the optimal conditions for that specific enzyme. |
| 1. | Both (A) and (R) are False. |
| 2. | Both (A) and (R) are True but (R) does not explain (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) explains (A). |
Subtopic: Process of Biotech |
54%
Level 3: 35%-60%
Hints
Identify the correct statement:
| 1. | DNA is the genetic material of all organisms without exception. |
| 2. | As it is an RNA world, the genetic material is ds RNA in majority of the organisms. |
| 3. | Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules. |
| 4. | Proteins such as histones can be removed from the cell lysate with the help of ribonuclease. |
Subtopic: Process of Biotech |
78%
Level 2: 60%+
Hints
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