Which of the enzymes whose recognition sites are shown here would produce sticky ends compatible with BstAUI (5'-G|GTACC-3')?

1. Bcl I (5'-T|GATCA-3')
2. Sun I (5'-C|GTACG-3')
3. Hind III (5'-A|AGCTT-3')
4. Kpn I (5'-GGTAC|C-3')

If you are using a vector with lacZ as a screenable marker, which of the following bacterial strains would you want to use as host cells?
1. lacO^-
2. lacI^-
3. lacZ^-
4. Any of these would work.

Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?
1. electroporation
2. liposome transfer
3. microinjection
4. particle bombardment

A fragment of DNA, cut by a restriction enzyme, forms bonds with other DNA molecules that have
1. Been fragmented by the same restriction enzyme
2. Sticky ends
3. Plasmid component
4. Attached methyl group

The DNA fragments separated on an agarose gel can be visualized after staining with:

1. Acetocarmine

2. Aniline blue

3. Ethidium bromide

4. Bromophenol blue

The process of separation and purification of expressed protein before marketing is called:

1. Downstream processing

2. Bioprocessing

3. Post-product processing

4. Upstream processing

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?

1. Eco Rl

2. taq polymerase

3. polymerase lll

4. ligase

Identify the incorrectly matched pair:

   restriction enzyme                   recognition site

    1. EcoRI                                5’GAATTC3’

                                                3’CTTAAG5’

     2. HindII                               5’GTPyPuAC3’

                                                3’CAPuPyTG3’

     3. SmaI                                5’GGATTC3’

                                                3’CTTAGG5’

    4. HindIII                              5’AAGCTT3’

                                                3’TTCGAA5’

Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactal bumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of rDNA technology.
(D) Disarmed pathogen vectors are also used in transfer of r-DNA into the host.
Which are the two statements having mistakes?
1. Statement (B) and (C)
2. Statement (C) and (D)
3. Statement (A) and (C)
4. Statement (A) and (B)

The introduction of t-DNA into plants involves:
1. Altering the pH of the soil, then heat shocking the plants
2. Exposing the plants to cold for a brief period
3. Allowing the plant roots to stand in water
4. Infection of the plant by Agrobacterium tumefaciens