Q. No.What is true for the plasmids used as cloning vectors in recombinant procedures?
1.
Ori is responsible for controlling the copy number of linked DNA
2.
The bacteria lacking R plasmids are resistant to antibiotics
3.
The vector must have many sites for the same restriction enzyme
4.
They can carry largest inserts amongst the available cloning vectors
What is true regarding A, B and C in the given diagrammatic representation of rDNA technology?
| I: | At A, same restriction enzyme is used to cut both foreign and vector DNA |
| II: | The enzyme used at B is DNA ligase |
| III: | Step C can be called as transformation |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II and III |
A and B in pBR322, shown in the diagram given below, respectively represent recognition sequences of:
2. Hind II and Sma I
3. BamH I and Sal I
4. Sal I and Hind I
What is true about the steps of PCR shown in the diagram given below?
| I: | Step A occurs at the temperature of 95 – 980 C |
| II: | Step B occurs at the temperature of 550 C |
| III: | Step C occurs at the temperature of 720 C |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II and III |
Which of the following primers would allow copying of the single-stranded DNA sequence 5' ATGCCTAGGTC?
| 1. | 5' TACGG | 2. | 5' CTGGA |
| 3. | 5' GACCT | 4. | 5' GGCAT |
The enzyme used in the polymerase chain reaction is a:
1. DNA dependent RNA polymerase
2. RNA dependent DNA polymerase
3. DNA dependent DNA polymerase
4. RNA dependent RNA polymerase
A host cell normally does not take up a foreign DNA until it has been made competent to do so. This is because:
| 1. | DNA is a hydrophilic molecule |
| 2. | DNA is a very large molecule |
| 3. | there are no receptors for DNA on the cell membrane |
| 4. | DNA is an inert molecule |
How does the bacterium protects its own DNA from the action of restriction endonucleases present in its cytoplasm?
| 1. | its DNA lacks active sites for the restriction endonucleases |
| 2. | its DNA is protected within a nuclear envelope |
| 3. | it adds methyl groups to the active sites of restriction endonucleases on its DNA |
| 4. | the DNA gets heterochromatised to prevent the action of restriction enzymes |
When “sticky ends” are paired, they can be joined by:
| 1. | restriction enzymes | 2. | polymerases |
| 3. | methylase | 4. | DNA ligase |
© 2024 GoodEd Technologies Pvt. Ltd.