Taq polymerase is used in the polymerase chain reaction because:
1. | It replicates DNA faster than other enzymes |
2. | It is the only enzyme that can replicate DNA invitro |
3. | It does not require an RNA primer to function |
4. | It is thermostable |
Who among the following was awarded the Nobel Prize for the development of the PCR technique?
1. | Herbert Boyer | 2. | Hargovind Khurana |
3. | Kary Mullis | 4. | Arthur Kornberg |
The chronological steps of a polymerase chain reaction are:
1. | Denaturation, Annealing of primers, Primer extension |
2. | Annealing of primers, Denaturation, Primer extension |
3. | Annealing of primers, Primer extension, Denaturation |
4. | Denaturation, Primer extension, Annealing of primers |
The enzyme used for DNA amplification in PCR procedures is:
1. Polynucleotide phosphorylase
2. Reverse transcriptase
3. Taq polymerase
4. DNA-dependent RNA polymerase