Elution is:
1. | separating the restricted DNA fragments on agarose gel |
2. | staining the separated DNA fragments with ethidium bromide |
3. | cutting out of the separated bands of DNA from the agarose gel and extracting them from the gel piece |
4. | constructing rDNA by joining the purified DNA fragments to the cloning vector |
Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
1. | wheat | 2. | soybean |
3. | rice | 4. | barley |
1. | 27°C | 2. | 42°C |
3. | 72°C | 4. | 98°C |
In microinjection, the rDNA :
1. | coats the micropellets and is used in biolistics to transform plant cells |
2. | is directly injected into the cytoplasm of a plant or an animal cell |
3. | is delivered in the vicinity of the plasma membrane of the cell (to be transformed) |
4. | is directly injected into the nucleus of an animal cell |
If you want to isolate DNA in pure form from a fungal cell, the first enzyme required normally would be:
1. | Restriction endonuclease | 2. | DNA ligase |
3. | DNA polymerase | 4. | Chitinase |