- 1(current)
Q. No.Elution is:
| 1. | separating the restricted DNA fragments on agarose gel |
| 2. | staining the separated DNA fragments with ethidium bromide |
| 3. | cutting out of the separated bands of DNA from the agarose gel and extracting them from the gel piece |
| 4. | constructing rDNA by joining the purified DNA fragments to the cloning vector |
Subtopic: Transforming Plant & Animal Cell | Obtaining Copy of Gene from Donor DNA |
83%
Level 1: 80%+
Hints
Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
| 1. | wheat | 2. | soybean |
| 3. | rice | 4. | barley |
Subtopic: Transforming Plant & Animal Cell |
63%
Level 2: 60%+
Hints
What temperature is used to give heat shock when competence is induced in a bacterial host cell enabling it to take up rDNA?
| 1. | 27°C | 2. | 42°C |
| 3. | 72°C | 4. | 98°C |
Subtopic: Transforming Plant & Animal Cell |
80%
Level 1: 80%+
Hints
In microinjection, the rDNA :
| 1. | coats the micropellets and is used in biolistics to transform plant cells |
| 2. | is directly injected into the cytoplasm of a plant or an animal cell |
| 3. | is delivered in the vicinity of the plasma membrane of the cell (to be transformed) |
| 4. | is directly injected into the nucleus of an animal cell |
Subtopic: Transforming Plant & Animal Cell | Host & Desired DNA |
78%
Level 2: 60%+
Hints
If you want to isolate DNA in pure form from a fungal cell, the first enzyme required normally would be:
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA polymerase | 4. | Chitinase |
Subtopic: Host & Desired DNA: I | Host & Desired DNA: II | Transforming Plant & Animal Cell |
88%
Level 1: 80%+
Hints
- 1(current)
Q. No.
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