- 1(current)
Q. No.
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Which of the following would NOT be a useful selectable marker?
| 1. | A gene encoding a protein that degrades the antibiotic ampicillin. |
| 2. | A gene encoding a protein that allows the cell to synthesize histidine. |
| 3. | A gene encoding a protein that is an essential structural component of the cell. |
| 4. | All of these are useful selectable markers. |
Subtopic: Â Selectable Markers: I |Â Selectable Markers: II |Â Selectable Markers |
Level 3: 35%-60%
Hints
You are inserting your gene of interest into the Lac Z gene in a plasmid also containing a tetracycline-resistant gene. You plate your transformed bacteria on media containing tetracycline and X-gal. Which of the following results would indicate a clone with recombinant plasmids?
| 1. | A clone which did not grow on the tetracycline plates |
| 2. | A white colony on the tetracycline plates |
| 3. | A blue colony on the tetracycline plates |
| 4. | A red colony on the tetracycline plates |
Subtopic: Â Selectable Markers: III |Â Selectable Markers |
 59%
Level 3: 35%-60%
Hints
Consider the following statements:
| I: | Ori is responsible for the copy number of the linked DNA. |
| II: | Transformation is a process through which a piece of DNA is introduced into a host bacterium. |
| III: | Bacteriophages have very high copy numbers of their genome within the bacterial cell. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Subtopic: Â Tools: Vectors: I |Â Selectable Markers: I |Â Selectable Markers |Â Tools |
 81%
Level 1: 80%+
Hints
- 1(current)
Q. No.
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