A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:

1.  Human genes may have intron which bacteria cannot process
2.  Amino acid codons for humans and bacteria are different
3.  Human protein is formed but degraded by bacteria
4.  All of the above

Subtopic:  Tools | General Design of an rDNA experiment |
 61%
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The enzyme DNA ligase, important in rDNA procedures, catalyzes the formation of:

1.N- Glycosidic2.Hydrogen
3.Phosphodiester4.Ester

Subtopic:  General Design of an rDNA experiment |
 62%
From NCERT
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A danger of genetic engineering is that the genetically modified bacterium can escape the lab and infect humans. This possibility is prevented by:

1.  selecting a mutant form of the bacteria
2.  selecting a live attenuated bacterium
3.  application of stringent asepsis in the lab
4.  prophylactically immunizing the persons working in the lab
Subtopic:  General Design of an rDNA experiment |
 59%
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