A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:
| 1. | Human genes may have intron which bacteria cannot process |
| 2. | Amino acid codons for humans and bacteria are different |
| 3. | Human protein is formed but degraded by bacteria |
| 4. | All of the above |
The enzyme DNA ligase, important in rDNA procedures, catalyzes the formation of:
| 1. | N- Glycosidic | 2. | Hydrogen |
| 3. | Phosphodiester | 4. | Ester |
A danger of genetic engineering is that the genetically modified bacterium can escape the lab and infect humans. This possibility is prevented by:
| 1. | selecting a mutant form of the bacteria |
| 2. | selecting a live attenuated bacterium |
| 3. | application of stringent asepsis in the lab |
| 4. | prophylactically immunizing the persons working in the lab |