Consider the following statements:
I: | Asexual reproduction preserves genetic information while sexual reproduction permits variations. |
II: | Traditional hybridization often leads to the inclusion and multiplication of undesirable genes along with the desired genes. |
III: | rDNA technology allows us to isolate and introduce only one or a set of desirable genes without introducing undesirable genes in the target organism. |
Which of the above statements are true?
1. | I and II only | 2. | I and III only |
3. | II and III only | 4. | I, II, and III |
What type of probe would you use for a Western blot experiment?
1. | A known DNA sequence. | 2. | An RNA molecule. |
3. | A purified protein. | 4. | An antibody. |
Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
1. | restriction endonuclease digestion of cellular and plasmid DNAs. |
2. | production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs. |
3. | separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates. |
4. | transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin. |
The advantage of using DNA polymerases from thermophilic organisms in PCR is that:
1. | the DNA polymerases of these bacteria are much faster than those from other organisms |
2. | the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands |
3. | the DNA polymerases of these bacteria never make mistakes while replicating DNA |
4. | all of the above |
In situ hybridization is used to :
1. | Remove mutations from a chromosome |
2. | Remove plasmids from a cell |
3. | Clone a gene |
4. | Locate a gene on a chromosome |
1. | The process of separation and purification of expressed protein before marketing is called as downstream processing |
2. | DNA precipitation out of a mixture of biomolecules can be achieved by treatment with a divalent cation such as calcium ion |
3. | Stirred-tank bioreactors have been designed for availability of oxygen throughout the process |
4. | Reporter genes can be used as markers for selecting successfully transformed cells |