Q. No.Nematode [Meloidogyne incognita] specific genes were transferred to tobacco plant using:
1.
Retroviral vectors
2.
Gene gun
3.
Agrobacterium mediation
4.
Micro-injection
Corn borer can be controlled by the proteins encoded by Bacillus thuringiensis gene:
| 1. | cryIAb | 2. | cryIAc |
| 3. | cryIIAb | 4. | cryIIIAc |
The name Genetic Engineering Approval Committee [GEAC] has been changed to:
| 1. | Genetic Engineering Advisory Committee |
| 2. | Genetic Engineering Appellate Committee |
| 3. | Genetic Engineering Appraisal Committee |
| 4. | Genetic Engineering Arbitration Committee |
ELISA is based on the principle of:
| 1. | Complementary hybridization of nucleic acids |
| 2. | Antigen-antibody interaction |
| 3. | Clonal selection |
| 4. | Southern blot |
Transgenic mice are being used to test the safety of polio vaccine. They could replace the use of:
| 1. | Monkeys | 2. | Rabbits |
| 3. | Fowl | 4. | Frogs |
The main challenge for production of insulin using rDNA techniques was:
| 1. | to find a suitable host cell that can perform RNA splicing |
| 2. | isolation of a copy of gene from the human DNA |
| 3. | getting insulin assembled into a mature form |
| 4. | downstream processing |
Alpha-1-antitrypsin was produced in a transgenic sheep, Tracy. This enzyme is used in the treatment of:
| 1. | Emphysema | 2. | Rheumatoid arthritis |
| 3. | Ovarian cancer | 4. | Pancreatitis |
Gene therapy for ADA deficiency has been tried with the help of which of the following vectors?
| 1. | Microinjection | 2. | Liposome |
| 3. | A bacterium | 4. | A retrovirus |
Identify the incorrect statement regarding the use of RNAi for creating transgenic tobacco plants resistant to Meloidogyne incognita:
| 1. | Agrobacterium tumefaciens was used to transform the plant cells |
| 2. | Double-stranded RNA was introduced into the plant cells |
| 3. | Double-stranded RNA was formed in the plant cells that initiated RNAi |
| 4. | A specific mRNA of the nematode was silenced with the help of dsRNA |
Why does the bacterium Bacillus thuringiensis not affected by the toxins produced by it that can kill arthropods?
| 1. | The toxin inactivated by proteins in the bacterial cells. |
| 2. | The bacterial cell does not have organelles and thus it is harmless. |
| 3. | It is produced as a pro-toxin by the bacterium. |
| 4. | The protein needs post-transcriptional modification possible only in eukaryotic cells. |
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