The first step to achieve somatic hybridization in plants will be:
1. | growing individual cells into callus |
2. | fusion of two cells in the presence of polyethylene glycol |
3. | converting plant cells into protoplast by cellulase |
4. | growing plant cells in cytokinin |
The name Genetic Engineering Approval Committee [GEAC] has been changed to:
1. | Genetic Engineering Advisory Committee |
2. | Genetic Engineering Appellate Committee |
3. | Genetic Engineering Appraisal Committee |
4. | Genetic Engineering Arbitration Committee |
ELISA is based on the principle of:
1. | Complementary hybridization of nucleic acids |
2. | Antigen-antibody interaction |
3. | Clonal selection |
4. | Southern blot |
Corn borer can be controlled by the proteins encoded by Bacillus thuringiensis gene:
1. | cryIAb | 2. | cryIAc |
3. | cryIIAb | 4. | cryIIIAc |
Nematode [Meloidogyne incognita] specific genes were transferred to tobacco plant using:
1. | Retroviral vectors | 2. | Gene gun |
3. | Agrobacterium mediation | 4. | Micro-injection |
Transgenic mice are being used to test the safety of polio vaccine. They could replace the use of:
1. | Monkeys | 2. | Rabbits |
3. | Fowl | 4. | Frogs |
The main challenge for production of insulin using rDNA techniques was:
1. | to find a suitable host cell that can perform RNA splicing |
2. | isolation of a copy of gene from the human DNA |
3. | getting insulin assembled into a mature form |
4. | downstream processing |
Alpha-1-antitrypsin was produced in a transgenic sheep, Tracy. This enzyme is used in the treatment of:
1. | Emphysema | 2. | Rheumatoid arthritis |
3. | Ovarian cancer | 4. | Pancreatitis |
Gene therapy for ADA deficiency has been tried with the help of which of the following vectors?
1. | Microinjection | 2. | Liposome |
3. | A bacterium | 4. | A retrovirus |
Identify the incorrect statement regarding the use of RNAi for creating transgenic tobacco plants resistant to Meloidogyne incognita:
1. | Agrobacterium tumefaciens was used to transform the plant cells |
2. | Double-stranded RNA was introduced into the plant cells |
3. | Double-stranded RNA was formed in the plant cells that initiated RNAi |
4. | A specific mRNA of the nematode was silenced with the help of dsRNA |