If a DNA fragment is cut by EcoRI at a specific recognition site then
1. Blunt-end cannot be obtained
2. Sticky-ends cannot be obtained
3. Construction of rDNA would not be possible
4. The plasmid with one site get cut into two fragments
The enzyme that seals 5’ PO4 and 3’ OH polynucleotide ends while creating a recombinant DNA molecule is
1. Alkaline phosphatase
2. DNA ligase
3. DNAse
4. Restriction endonuclease
Part of a plasmid such as pBR322 responsible for controlling the copy number of the linked alien DNA is
1. Ori
2. rop
3. ampR
4. tetR
DNA from Agrobacterium tumefaciens can be extracted through use of enzyme
1. Chitinase
2. Cellulase
3. Lysozyme
4. DNAse
Select the incorrect statement.
1. Separation of DNA fragments occurs based on their size in agarose gel.
2. Blue-white selection involves insertional inactivation of β-galactosidase.
3. Treatment with ice-cold calcium can enhance efficiency of transformation in host cells.
4. Extension step during PCR is based on thermolabile nature of Taq polymerase.
All the following are transformation procedures except
1. Microinjection
2. Biolistics
3. Spooling
4. Electroporation
Downstream processing does not involve
1. Biosynthesis
2. Separation
3. Purification
4. Centrifugation
If a foreign gene is inserted into pBR322 by using restriction enzyme BamHI, the selection process, for identifying recombinants requires plating on all except
1. Ampicillin rich medium
2. Tetracycline rich medium
3. X-gal rich medium
4. Nutrient medium without antibiotics
If any protein-encoding gene is expressed in a heterologous host, it is termed as
1. Recombinant protein
2. Single-cell protein
3. Humulin
4. Transposon
Read the following statements
Statement-A : Restriction enzymes are obtained only from eukaryotes.
Statement-B : The first discovered restriction endonuclease was EcoRI.
Choose the correct option.
(1) Both statements are correct
(2) Both statements are incorrect
(3) Statement A is correct but B is incorrect
(4) Statement B is correct but A is incorrect