Which of the following are used in gene cloning:
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleoids
In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:
A. | a bacterium | B. | plasmid |
C. | plasmodium | D. | bacteriophage |
Options:
1. | (A) only | 2. | (A) and (C) only |
3. | (B) and (D) only | 4. | (A), (B) and (D) only |
In genetic engineering, the antibiotic-resistance gene is used:
1. To select healthy vectors
2. As sequences from where replication starts
3. To keep the cultures free of infection
4. As selectable markers
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
1. | Dark red bands | 2. | Bright blue bands |
3. | Yellow bands | 4. | Bright orange bands |
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
1. Extension, Denaturation, Annealing
2. Annealing, Denaturation, Extension
3. Denaturation, Annealing, Extension
4. Denaturation, Extension, Annealing
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
1. | Histones | 2. | Polysaccharides |
3. | RNA | 4. | DNA |
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain,
1. | it will lead to the lysis of host cells. |
2. | it will be able to produce a novel protein with dual abilities. |
3. | it will not be able to confer ampicillin resistance to the host cell. |
4. | the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside. |
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1. Palindromic Nucleotide sequences
2. Poly(A) tail sequences
3. Degenerate primer sequence
4. Okazaki sequences
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
1. | Denaturation | 2. | Ligation |
3. | Annealing | 4. | Extension |
During the process of gel electrophoresis, DNA moves towards the __ of the electrophoretic chamber as the charge of DNA is __.
Choose the option that fills the blanks correctly.
A B
1. Cathode Positive
2. Cathode Negative
3. Anode Positive
4. Anode Negative