Q. No.During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
1.
Histones
2.
Polysaccharides
3.
RNA
4.
DNA
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain,
| 1. | it will lead to the lysis of host cells. |
| 2. | it will be able to produce a novel protein with dual abilities. |
| 3. | it will not be able to confer ampicillin resistance to the host cell. |
| 4. | the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside. |
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1. Palindromic Nucleotide sequences
2. Poly(A) tail sequences
3. Degenerate primer sequence
4. Okazaki sequences
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
| 1. | Denaturation | 2. | Ligation |
| 3. | Annealing | 4. | Extension |
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
1. Extension, Denaturation, Annealing
2. Annealing, Denaturation, Extension
3. Denaturation, Annealing, Extension
4. Denaturation, Extension, Annealing
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
| 1. | Dark red bands | 2. | Bright blue bands |
| 3. | Yellow bands | 4. | Bright orange bands |
In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:
| A. | a bacterium | B. | plasmid |
| C. | plasmodium | D. | bacteriophage |
Options:
| 1. | (A) only | 2. | (A) and (C) only |
| 3. | (B) and (D) only | 4. | (A), (B) and (D) only |
Which of the following are used in gene cloning:
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleoids
In genetic engineering, the antibiotic-resistance gene is used:
1. To select healthy vectors
2. As sequences from where replication starts
3. To keep the cultures free of infection
4. As selectable markers
| Assertion (A): | Polymerase chain reaction is used in DNA amplification. |
| Reason (R): | The ampicillin resistant gene is used as a selectable marker to check transformation. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is not correct but (R) is correct. |
| 2. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 3. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
| 4. | (A) is correct but (R) is not correct. |
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