Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?
1. BamHI
2. Psti
3. Clal
4. Pvull
The technique not used for transformation of plant cells in recombinant procedures is:
1. Biolistics
2. Agrobacterium mediation
3. Use of viruses
4. Micro-injection
In the screening process during rDNA experiments, clones that metabolize -gal turn:
1. Colorless
2. Blue
3. Yellow
4. Green
Microinjection is suitable for :
1. Injecting an ovum into the sperm in IVF
2. Transforming animal cells
3. Injecting very small sized drug particles into neurons
4. Conferring antibiotic resistance to a certain strain of bacteria
The plasmid pBR322 does not contain:
1. An origin of replication site
2. A gene that encodes for restrictor of plasmid copy number
3. Gene for Ampicillin and Streptomycin resistance
4. Sites for many restriction enzymes
The bioreactor shown in the diagram is:
1. Sparged stirred tank type
2. Airlift type
3. Simple stirred tank type
4. Fluidized bed type
What is true about the steps of PCR shown in the diagram given below?
l. Step A occurs at the temperature of 95-98 C
ll. Step B occurs at the temperature of 55 C
lll. Step C occurs at the temperature of 72 C
1. l and ll only
2. l and lll only
3. ll and lll only
4. l, ll and lll
The process of separation and purification of expressed protein before marketing is called
(1) upstream processing
(2) downstream processing
(3) bioprocessing
(4) postproduction processing
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?
(1) The larger the fragment size, the farther it moves
(2) The smaller the fragment size, the farther it moves
(3) Positively charged fragments move to farther end
(4) Negatively charged fragments do not move
A gene, whose expression helps to identify transformed cells is known as
1. selectable marker
2. vector
3. plasmid
4. structural gene