Which of the following is not correctly matched for the organism and its cell wall
degrading enzyme?
1. Bacteria —Lysozyme
2. Plant cells — Cellulase
3. Algae —Methylase
4. Fungi — Chitinase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
(1) Non-recombinant bacteria containing -galactosidase
(2) Insertional inactivation of -galactosidase in non-recombinant bacteria
(3) Insertional inactivation of β-galactosidase in recombinant bacteria
(4) Inactivation of glycosidase enzyme in recombinant bacteria
Which one is a true statement regarding DNA polymerase used in PCR?
1. It is used to ligate introduced DNA in recipient cells
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature
For transformation, microparticles coated with DNA to be bombarded with gene gun are
made up of
1. silver or platinum
2. platinum or zinc
3. silicon or platinum
4. gold or tungsten
Given below is a sample of portion of DNA strand giving the base sequence on the
opposite strands. What is so, special shown in it?
5'__ GAATTC_ 3'
3' __ CTTAAG__5'
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?
1. Coelom
2. Coenzyme
3. coli
4. Colon
Agarose extracted from sea weeds finds use in
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
Which one of the following is used as vector for cloning genes into higher organisms?
1. Baculovirus
2. Salmonella typhimurium
3. Rhizopus nigricans
4. Retrovirus
Which one of the following palindromic base sequences in DNA can be easily cut at about
the middle by some particular restriction enzyme?
1. 5'CGTTCG3'
3'ATGGTA-5'
2. 5'GATATG3'
3'CTACTA5'
3. 5'GAATTC3'
3'CTTAAG5'
4. 5'CACGTA3'
3'CTCAGT5'
Which one of the following is commonly used in transfer of foreign DNA into crop plants?
1. Trichoderma harzianum
2. Meloidogyne incognita
3. Agrobacterium tumefaciens
4. Penicillium expansum