The source of taq polymerase used in PCR is a
(1) thermophilic fungus
(2) mesophilic fungus
(3) thermophilic bacterium
(4) halophilic bacterium
Which of the following statement is not true:
(1) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium
(2) Normally, the genes encoding resistance to antibiotics are considered useful selectable markers for E.coli
(3) The normal E.coli cells do not carry resistance against antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin
(4) Presence of one recognition sites within the vector will generate several fragments, which will complicate the gene cloning
To be useful in genetic engineering a vector must have
In 1960’s two enzymes were discovered in bacteria that were responsible for providing immunity against bacteriophages. One was Restriction Endonuclease and the other was
1. Methylase
2. Exonuclease
3. Aminotransferase
4. Terminal Transferase
E.coli is a commonly used host for gene cloning because
1. It is free from elements that interfere with replication and recombination of DNA
2. It is easy to transform
3. It supports replication of inserted DNA.
4. All of these
A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called
a. Suppression vector
b. Expression vector
c. Cloning vector
d. None of these
The names associated with construction of first Recombinant DNA are
a. Arber, Nathans, Smith
b. Annie Chang, Boyer, Berg, Cohen
c. Howard Temin, Brenner, Sharp
d. Tim Hunt, Hartwell, Nurse
Insertional inactivation of a gene helps in
a. Identification of recombinant clones
b. Identification of deletion mutants
c. Identification of recombinant transformants
d. Elimination of suppression mutants
What is true about the DNA polymerase used in PCR?
(1) Isolated from a virus
(2) Stable at high temperatures
(3) Used as selectable marker
(4) Has methylated bases
Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
a. Zinc or tungsten
b. Silicon or gold
c. Tungsten or gold
d. Selenium or Platinum